Biopolym. Cell. 1996; 12(6):63-68.
Molecular-biology approaches to detection of Philadelphia chromosome in patients with leukemia
1Telegeev G. D., 1Dybkov M. V., 1Bozhko M. V., 2Tretiak N. M., 1Maliuta S. S.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Kyiv Institute of Hematology and Blood Transfusion, MoH Ukraine
    street. Maxim Berlinskogo, 12., Kyiv, Ukraine, 252060


The data on Philadelphia chromosome detection in patients with CML and ALL are presented. Two approaches were applied: .5' and 3' bcr genomic probes were used; two steps PCR with specific primers was employed. The latter is more preferable for clinic use. Some issues of Ph' leukemias etiology are also discussed.


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