Biopolym. Cell. 1996; 12(6):69-73.
Characterization of catalytic activity in vitro of the ribozyme targeted to tat-RNA of HIV-I
1Buryanovsky L. N., 1Shved A. D.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Essential properties of catalytic activity in vitro of the anti-tat RNA (HIV-I) hammerhead ribozyme have been studied. Spermidine-dependencies, discovered by us earlier, has supported. Optimal concentration of magnesium ion were established and was equal 5–10 mM. Temperature optimum of reaction was 30 °C. 20 % formamide has stimulating influence on efficiency of the cleavage at 15 °C. Time-dependence course of efficiency of reaction also were carried out.


[1] Kruger K, Grabowski PJ, Zaug AJ, Sands J, Gottschling DE, Cech TR. Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena. Cell. 1982;31(1):147-57.
[2] Hutchins CJ, Rathjen PD, Forster AC, Symons RH. Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid. Nucleic Acids Res. 1986;14(9):3627-40.
[3] Uhlenbeck OC. A small catalytic oligoribonucleotide. Nature. 1987 Aug 13-19;328(6131):596-600.
[4] Haseloff J, Gerlach WL. Simple RNA enzymes with new and highly specific endoribonuclease activities. Nature. 1988;334(6183):585-91.
[5] Buryanovsky LN, Schved AD. In vitro specific cleavage of HIV-1 tat-RNA by catalytically active polyribonucleotide, ribozyme Biopolym. Cell. 1996; 12(2):20-23
[6] Denman RB. Cleavage of full-length beta APP mRNA by hammerhead ribozymes. Nucleic Acids Res. 1993;21(17):4119-25.
[7] Pachuk CJ, Yoon K, Moelling K, Coney LR. Selective cleavage of bcr-abl chimeric RNAs by a ribozyme targeted to non-contiguous sequences. Nucleic Acids Res. 1994;22(3):301-7.
[8] Sun LQ, Wang L, Gerlach WL, Symonds G. Target sequence-specific inhibition of HIV-1 replication by ribozymes directed to tat RNA. Nucleic Acids Res. 1995;23(15):2909-13.