Biopolym. Cell. 1996; 12(6):74-81.
Interaction of cyanine dyes with nucleic acids. 2. Spectroscopic properties of methyleneoxy analogues of Thiazole Orange
1Yarmoluk S. M., 1Kovalska V. B., 2Smirnova T. V., 2Shandura M. P., 2Kovtun Y. P., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine
    1, Murmans'ka Str., Kyiv, Ukraine, 02094


A series of asymmetric cyanine dyes based on methylenoxy benzothiazole terminal hetero-cycle was synthesized. Absorption and fluorescent properties of these dyes and their complexes with nucleic acids were investigated. The best results were obtained with 2-[(3-methyl-2(3H)-benzothiazolyliden)-methyl]-5,6-dioxymethylene-3-methylbenzothiaz olium p-toluenesulfonate (Cyan 13). A possible model of binding monomethyne benz-thiazole cyanine dyes with double-stranded nucleic acids is proposed.


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