Biopolym. Cell. 1997; 13(3):232-238.
Molecular Mechanisms of Differentiation
Optimization of the system for emhryogenetic studies on inbred strains of mice. 3. Conditions of hormonal stimulation of females and in vitro culture of preimplanlation embryos of ICR, CBA/Lac and C57Bl/6j mouse strains
1Vagyna I. N., 1Titok T. G., 1Sarapina N. A., 1Kirichenko Y. V., 1Evsikov S. V., 1Solomko A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Optimal conditions for hormonal stimulation of females of ICR, CBAlLac and C57Bl/6j have bean worked out The optimal concentration and spectrum of hormones for each strain was determined. Conditions for in vitro culture of zygotes of different strains were elaborated. It was determined that the important thing for successive in vitro culture of zygotes is the stage of development of fertilized ova, which is characterized by the time of appearance of maternal and paternal pronuclei.


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