Biopolym. Cell. 1997; 13(4):308-313.
Genome and Its Regulation
Transforming growth factor a production, EGF receptor level and expression of transformed phenotype in HEp-2 human carcinoma cells transfected with HIV-1 tat gene
1Phylchenkov A. A., 2Yakimovich I. A., 2Korchinsky A. G., 1Kudryavels Yu. I., 2Stoika R. S.
  1. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022
  2. Division of Cell Regulatory Systems of O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005


Several clones of HEp-2 human carcinoma cells transfected with HIV-1 lot regulatory gene were studied for the presence of transforming growth factor a (TGF-α) activity in the serum-free culture medium conditioned by these cells, the level of epidermal growth factor (EGF)lTGF-α receptors and the expression of transformed phenotype. Clone A5st was shown to secrete growth stimulating factors whose activity was inhibited by the monoclonal antibody against TGF-α. The cells of alt studied clones possessed the same level of EGF/ TGF-α receptors measured by two different, methods – indirect flow cytometry and radioreceptor analysis. The expression of transformed phenotype (estimated on the basis of intensity of colonyforming by the cells cultured in the medium supplemented with 0.33 % agar) did not change under the effect of EGF in A5st clone producing TGF-α immunoreactive factors. But this expression was induced in the other studied clones of HEp-2 cells. Thus, the expression of HIV-1 tat regulatory gene can induce cellular production of TGF-α which participates in the cell transformation and in this way increases the risk of development of oncological diseases in AIDS patients.


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