Biopolym. Cell. 1997; 13(5):397-402.
Genome and Its Regulation
Structure rearrangement of plasmid pATV8 in transgenic mice. 2. Physical mapping of autonomous transgenome and sequencing of cloned fragment of its modified region
1Chashchina L. I., 1Krysan K. V., 1Matkovsky V. I., 1Solomko A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The existence of extrachromosomal transgenome in several substrains of transgenic mice was shown. This transgenome is a modified pATV8 plasmid. Analysis of its structure and biological properties demonstrated that the ori and ampicillin resistance regions of bacterial part of pATV8 did not change, but the proviral part of initial plasmid had severely modified. Partial elimination of proviral DNA and transposition of new sequence occured. It seems that stable mitotic and meiotic segregation of exlrachromosomal transgenome and its stable replication depends upon the acquired fragment of mouse genomic DNA.


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