Biopolym. Cell. 1999; 15(1):38-42.
Cell Biology
Morphometric characteristic of cell culture L929 after the termic and radiation action
1Baraboy V. A., 1Tanzura T. V., 1Kovalenko L. S., 1Zinchenko V. A., 1Lawentchuk G. J.
  1. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
    45, Vasilkivska Str., Kyiv, Ukraine, 01022


Using morphometric indices (cell, nucleus and nucleoli surface square) the influence of the transforming agents such as methyl-chalanthrene, X-ray (15 Gy) and hyperthermia (40 and 43 °C) separately and in different combinations was studied. The aim of this investigation is a finding of optimal scheme of tumor thermoradiotherapy. It was shown that combination of hyperthermia (40 or 43 °C) together with irradiation destructed cell the most.


[1] Overgaard J. The rationale for clinical trials in hiperthermia /V An Introduction to the practical aspects of clinical hyperthermia. London; New York, 1990:213—241
[2] Zhavrid E. A., Osinskiy S. P., Fradkin S Z. Hyperthermia and hyperglycemia in oncology. Naukova Dumka 1987. 256.
[3] Schorcht J. Hyperthermie in der Tumortherapie. Radio biol. Radiother. 1990. 31, N 5:383—390.
[4] Konings A. W. T. Radiosensitization by hyperthermia: Mei-Iianisme of action. Radiation Research 1895—1995: Prot Tenth Int. Congr. of Radiation Research / Lds U. Hagen, D Harder, H. Yang. Wurzburg, 1995,Vol. 2:989--993.
[5] Ganul V. L., Baraboy B. A., Zinchenko V. A., Segeda T. P. Cytokinetic response induced by DMBA tumors in rats at different variants thermoradiotherapy. Vopr. onkologii.1992. 35(11):1376-1379.
[6] Baraboy V. A., Zinchenko V. A., Kovalenko L. S., Segeda T. P. Influence of various schemes of thermoradiotherapy on kinetic performance rhabdomyosarcoma cell sarcoma 45 in rats and cell culture Hep-2. Eksp. onkol.1993. 15(4):70—74.
[7] Baraboy V. A., Bobro L. I., Zinchenko V. A. Pathomorphosis and proliferative activity of rat rhabdomyosarcoma with thermal and radiation exposure. Klin. onkologiya. 1992; 12:62—67.
[8] Kalendo G. S. Early response of cells to ionizing radiation and their role in the protection and sensitization. M Energoizdat, 1982. 96p.
[9] Lavrenchuk G. I. Study the viability of transformed fibroblasts and differentiation of myogenic cells exposed to the density and radiation redkoioniziruyushey: Avtoref. diss. ... kand. biol. nauk. Kiyev, 1988. 20 s.
[10] Tashke K. Introduction to quantitative cyto histological morphology.Bucharest Academy of Soc. Resp. Romania, 1980. 191 p.
[11] Field S. B. In vivo aspects of hyperthermic oncology. Introduction to the practical aspects of clin. hyoerthermia / Eds S. B. Field, Y. W. Hand. London; New York: Philad, Taylor and Francis, 1990:55—68.