Biopolym. Cell. 1999; 15(1):49-56.
Cell Biology
Factors affecting the viability of the bovine embryos produced in vitro
1Kuznetsova I. B., 1Kuznetsov V. E., 1Kovtun S. I.
  1. Institute of animals breeding and genetics, UAAS
    1, Pogrebnjaka Str., p. Chubinskoe, Boryspil district, Kiev region., Ukraine, 08321


It is discussed the effects of different factors on the in vitro maturation and fertilization of cow oocytes, early embryonic development to the blastocyst stage and morphological and biochemical similarities and differences between embryos produced in vitro or in vivo.


[1] Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF, Dressel MA. Normal development following in vitro fertilization in the cow. Biol Reprod. 1982;27(1):147-58.
[2] Hinrichs K. Manipulation of oocyte maturation in vitro. Arch. Tierz. Dummerstorf. 1996. 39, Special Issue:43—50.
[3] Goto K, Kajihara Y, Kosaka S, Koba M, Nakanishi Y, Ogawa K. Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes. J Reprod Fertil. 1988;83(2):753-8.
[4] Galli C., Lazzari G. Large scale production of bovine embryos. Proc. Eur. Conf. Embryo Techn. and Genet Engin. In Cattleand Sheep. Krakow, 1994:111 — 116.
[5] Sirard MA, Blondin P. Oocyte maturation and IVF in cattle. Anim Reprod Sci. 1996; 42(1):417—426
[6] Konishi M, Aoyagi Y, Takedomi T, Itakura H, Itoh T, Yazawa S. Presence of granulosa cells during oocyte maturation improved in vitro development of IVM-IVF bovine oocytes that were collected by ultrasound-guided transvaginal aspiration. Theriogenology. 1996;45(3):573-81.
[7] van Inzen WG, van Stekelenburg-Hamers AE, Weima SM, Kruip TA, Bevers MM, Mummery CL. Culture of bovine embryos to the blastocyst stage using Buffalo rat liver (BRL) cells. Theriogenology. 1995;43(4):723-38.
[8] Saumande J. Culture of bovine granulosa cells in a chemically defined serum-free medium: the effect of insulin and fibronectin on the response to FSH. J Steroid Biochem Mol Biol. 1991;38(2):189-96.
[9] O'Doherty EM, Wade MG, Hill JL, Boland MP. Effects of culturing bovine oocytes either singly or in groups on development to blastocysts. Theriogenology. 1997;48(1):161-9.
[10] Adams R. Methods of cell culture for biochemists. M.: Mir, 1983. 258 p.
[11] Gordon I. In vitro maturation (IVM) and fertilization (IVF) of cattle ova. Embryo Transfer Newsletter. 1990. 8:6—11.
[12] Menard DP, Lamothe P, Smith LC. Aspiration transvaginale d'ovocytes chez les bovins: mise au point et evaluation d'une technique. Med. Vet. Quebec. 1995. 25:92-96.
[13] Blondin P, Guilbault LA, Sirard MA. In vitro production of bovine embryos: Developmental competence is acquired before maturation. 1995; 43(1):168
[14] Parrish JJ, Susko-Parrish JL, First NL. Effect of heparin and chondroitin sulfate on the acrosome reaction and fertility of bovine sperm in vitro. Theriogenology. 1985. 24O(5):537—549.
[15] Parrish JJ, Susko-Parrish JL, Handrow RR, Sims MM, First NL. Capacitation of bovine spermatozoa by oviduct fluid. Biol Reprod. 1989;40(5):1020-5.
[16] Gordon I. Large-scale production of cattle embryos by in vitro culture methods. Agr. Biotech. News and Inform. 1989. 1(3):345—348.
[17] Katska L, Smorag Z. Bull effect on in vitro embryo production in cattle. J. Physiol. Pharmacol. 1996. 47(2):71 —78.
[18] Miller GF, Gliedt DW, Rakes JM, Rorie RW. Addition of penicillamine, hypotaurine and epinephrine (PHE) or bovine oviductal epithelial cells (BOEC) alone or in combination to bovine in vitro fertilization medium increases the subsequent embryo cleavage rate. Theriogenology. 1994;41(3):689-96.
[19] Lee ES, Fujii Y, Fukui Y. A comparative study on developmental capacity to blastocysts derived from 1-and 2(3)-cell bovine embryos after in vitro maturation and fertilization. Theriogenology. 1996;45(6):1151-62.
[20] Reed WA, Suh TK, Bunch TD, White KL. Culture of in vitro fertilized bovine embryos with bovine oviductal epithelial cells, Buffalo rat liver (BRL) cells, or BRL-cell-conditioned medium. Theriogenology. 1996;45(2):439-49.
[21] Thibodeaux JK, Del Vecchio RP, Hansel W. Role of platelet-derived growth factor in development of in vitro matured and in vitro fertilized bovine embryos. J Reprod Fertil. 1993;98(1):61-6.
[22] Donnay I, Van Langendonckt A, Auquier P, Grisart B, Vansteenbrugge A, Massip A, Dessy F. Effects of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology. 1997;47(8):1549-61.
[23] Gandolfi F. Autocrine, paracrine and environmental factors influencing embryonic development from zygote to blastocyst. Theriogenology. 1994. 41(1):95—100.
[24] Thompson JG. Comparison between in vivo-derived and in vitro-produced pre-elongation embryos from domestic ruminants. Reprod Fertil Dev. 1997;9(3):341-54.
[25] Berg U, Brem G. In vitro Production of Bovine Blastocysts by in vitro Maturation and Fertilization of Oocytes and Subsequent in vitro Culture. Reprod Domest Anim (Zuchthygiene). 1989. 24(3):134—139.
[26] Carnegie JA, Durnford R, Algire J, Morgan J. Evaluation of mitomycin-treated vero cells as a co-culture system for IVM/IVF-derived bovine embryos. Theriogenology. 1997;48(3):377-89.
[27] Katska L, Rynska B, Smorag Z. The effect of co-culture system on developmental capacity of bovine IVM/IVF oocytes. Theriogenology. 1995;43(5):859-70.
[28] Katska L. Hodowla zarodkow ssakow in vitro. Med. Wet. 1996.11:6.
[29] Keskintepe L, Brackett BG. In vitro developmental competence of in vitro-matured bovine oocytes fertilized and cultured in completely defined media. Biol Reprod. 1996;55(2):333-9.
[30] Takahashi Y, First NL. In vitro development of bovine one-cell embryos: Influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology. 1992;37(5):963-78.
[31] Mermillod P, Vansteenbrugge A, Wils C, Mourmeaux JL, Massip A, Dessy F. Characterization of the embryotrophic activity of exogenous protein-free oviduct-conditioned medium used in culture of cattle embryos. Biol Reprod. 1993;49(3):582-7.
[32] Rosenkrans CF Jr, Zeng GQ, MCNamara GT, Schoff PK, First NL. Development of bovine embryos in vitro as affected by energy substrates. Biol Reprod. 1993;49(3):459-62.
[33] Kim JH, Funahashi H, Niwa K, Okuda K. Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium. Theriogenology. 1993;39(4):875-86.
[34] Lim JM, Okitsu O, Okuda K, Niwa K. Effects of fetal calf serum in culture medium on development of bovine oocytes matured and fertilized in vitro. Theriogenology. 1994;41(5):1091-8.
[35] Shamsuddin M, Larsson B, Gustafsson H, Rodriguez-Martinez H. A serum-free, cell-free culture system for development of bovine one-cell embryos up to blastocyst stage with improved viability. Theriogenology. 1994;41(5):1033-43.
[36] Kruip TAM, den Daas JHG. In vitro produced and cloned embryos: Effects on pregnancy, parturition and offspring. Theriogenology. 1997;47(1):43—52.
[37] Walker SK, Hartwich KM, Seamark RF. The production of unusually large offspring following embryo manipulation: Concepts and challenges. Theriogenology. 1996; 45(1):111—120.
[38] Massip A, Mermillod P, Dinnyes A. Morphology and biochemistry of in-vitro produced bovine embryos: implications for their cryopreservation. Hum Reprod. 1995;10(11):3004-11.
[39] Avery B, Madison V, Greve T. Sex and development in bovine in-vitro fertilized embryos. Theriogenology. 1991;35(5):953-63.
[40] Dominko T, First NL. Relationship between the maturational state of oocytes at the time of insemination and sex ratio of subsequent early bovine embryos. Theriogenology. 1997;47(5):1041-50.
[41] Bredbacka K, Bredbacka P. Glucose controls sex-related growth rate differences of bovine embryos produced in vitro. J Reprod Fertil. 1996;106(2):169-72.