Biopolym. Cell. 2000; 16(1):40-45.
Structure and Function of Biopolymers
Comparative study of troponin complex immunoreactivity at dilated and ischemic cardiomyopathies
1Sidorik L. L., 1Fedorkova O. M., 2Ryabenko D. V., 1Bobyk V. I., 3Danilova V. M., 3Tregubov V. S., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Scientific Center "M. D. Strazhesko Institute of Cardiology, MAS of Ukraine"
    5, Narodnogo Opolchennya Str., Kyiv, Ukraine, 03151
  3. Petr Bogach Institute of Physiology
    Taras Shevchenko National University of Kyiv
    2, Academika Glushkova Ave Str., Kyiv, Ukraine, 03187


The features of the immune reactions against troponin complex isolated from human ventricular myocardium in sera of patients with dilated cardiomyopathy (DCM) and patients with cardiomegaly of ischemic origin (ICM) have been investigated. The results have shown that sera antibodies level against troponin complex antigenes were considerably higher in patients with DCM. The most pronounced immunogenisity of troponin complex isolated from DCM patient myocardium has been shown.


[1] Amosova EYa. Cardiomyopathy. Manual. K.: Kniga plyus, 1999; 425 p.
[2] Caforio AL. Role of autoimmunity in dilated cardiomyopathy. Br Heart J. 1994;72(6 Suppl):S30-4.
[3] Goldman JH, McKenna WJ. Immunopathogenesis of dilated cardiomyopathies. Curr Opin Cardiol. 1995;10(3):306-11.
[4] Caforio AL, Grazzini M, Mann JM, Keeling PJ, Bottazzo GF, McKenna WJ, Schiaffino S. Identification of alpha- and beta-cardiac myosin heavy chain isoforms as major autoantigens in dilated cardiomyopathy. Circulation. 1992;85(5):1734-42.
[5] Caforio AL, Keeling PJ, Mestroni L. Increased frequency of organ-specific cardiac antibodies in healthy relatives of patients with dilated cardiomyopathy: evidence for autoimmune pathogenesis. Br Heart J. 1993; 6.(Abstr.):7.
[6] Latif N, Baker CS, Dunn MJ, Rose ML, Brady P, Yacoub MH. Frequency and specificity of antiheart antibodies in patients with dilated cardiomyopathy detected using SDS-PAGE and western blotting. J Am Coll Cardiol. 1993;22(5):1378-84.
[7] Konstadoulakis MM, Kroumbouzou H, Tsiamis E, Trikas A, Toutouzas P. Clinical significance of antibodies against tropomyosin, actin and myosin in patients with dilated cardiomyopathy. J Clin Lab Immunol. 1993;40(2):61-7.
[8] Sidorik LL, Fedorkova OM, Kovenja TV, Bobyk VI, Rodnin NV, Matsuka GKh. Structural and functional studies of cardiac tropomyosin as; autoantigen at dilated cardiomyopathy. Biopolym Cell. 1998; 14(3):203-9.
[9] Sidorik LL Ryabenko DV, Bobyk VI, Rodnin NV, Ovcharenko GV, El'skaya AV, Matsuka GKh. Immune response to certain intracellular antigens in patients with dilated cardiomyopathy. Ukr Cardiol Zh. 1999; (1): 46-51.
[10] Cardiomyopathy: Proc WHO Expert Committee: Series tehn. rep .: 697. Geneva, 1985.
[11] Barskaia NV, Gusev NB. [Troponin from th bovine heart: isolation and study of cation-binding properties using a 3-dimethylaminonaphtheurodine fluorescent probe]. Biokhimiia. 1981;46(3):495-503.
[12] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
[13] Sobieszek A. Gradient polyacrylamide gel electrophoresis in presence of sodium dodecyl sulfate: a practical approach to muscle contractile and regulatory proteins. Electrophoresis. 1994;15(8-9):1014-20.
[14] Matsiota P, Druet P, Dosquet P, Guilbert B, Avrameas S. Natural autoantibodies in systemic lupus erythematosus. Clin Exp Immunol. 1987;69(1):79-88.
[15] Hunkeler NM, Kullman J, Murphy AM. Troponin I isoform expression in human heart. Circ Res. 1991;69(5):1409-14.
[16] Anderson PA, Malouf NN, Oakeley AE, Pagani ED, Allen PD. Troponin T isoform expression in humans. A comparison among normal and failing adult heart, fetal heart, and adult and fetal skeletal muscle. Circ Res. 1991;69(5):1226-33.
[17] Solaro RJ, Rarick HM. Troponin and tropomyosin: proteins that switch on and tune in the activity of cardiac myofilaments. Circ Res. 1998;83(5):471-80.