Biopolym. Cell. 2000; 16(2):108-114.
Structure and Function of Biopolymers
Subrepeats of the intergenic spacer of the Nicotiana ribosomal DNA
1Komarnytsky S. I.
  1. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680


Restriction analysis of nuclear DNA combined with, the blot-hybridization has been used to determine the distribution of the subrepeats of central part of Nicotiana tabacum rDNA intergenic spacer (IGS) among 68 Nicotiana tabacum species. It has been shown that the ancestor of N. plumbaginifolia or N. longiflora may be the donor of N. clevelandii (Bigelovianae), N. debneyi and N. suaveolens (Suaveolentes) rDNAs. N. corymbosa, N. miersii or N. spegazzinii may be the second nuclear DNA donor for N. debneyi, whereas N. acaulis may have played this role in the case of N. suaveolens.


[1] Long EO, Dawid IB. Repeated genes in eukaryotes. Annu Rev Biochem. 1980;49:727-64.
[2] Hemleben V, Ganal M, Gerstner J, Schiebel K, Torres RA. Organization and length heterogeneity of plant ribosomal RNA genes. The Architecture of Eukaryotic Genes. Weinheim: VHC, 1988: 371-84.
[3] Schiebel K, von Waldburg G, Gerstner J, Hemleben V. Termination of transcription of ribosomal RNA genes of mung bean occurs within a 175 bp repetitive element of the spacer region. Mol Gen Genet. 1989;218(2):302-7.
[4] Kato A, Yakura K, Tanifuji S. Repeated DNA sequences found in the large spacer of Vicia faba rDNA. Biochim Biophys Acta. 1985;825(4):411–5.
[5] Unfried K, Schiebel K, Hemleben V. Subrepeats of rDNA intergenic spacer present as prominent independent satellite DNA in Vigna radiata but not in Vigna angularis. Gene. 1991;99(1):63-8.
[6] Goodspeed TH. The genus Nicotiana. Massachusetts: Wal-tham, 1954. 536 p.
[7] Kostoff D. Cytogenetics of the genus Nicotiana. Karyosys-tematics, genetics, cytology, cytogenetics and phylesis of tobaccos. Sofia: State Printed House, (1941-43). 1071 p.
[8] Gray JC, Kung SD, Wildman SG. Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein. Nature. 1974;252(5480):226-7.
[9] Gray JC. Serological reactions of Fraction I proteins from interspecific hybrids in the genusNicotiana. Plant Syst Evol. 1978;129(3):177–83.
[10] Salts Y, Herrmann RG, Peleg N, Lavi U, Izhar S, Frankel R, Beckmann JS. Physical mapping of plastid DNA variation among eleven Nicotiana species. Theor Appl Genet. 1984;69(1):1-14.
[11] Kung SD, Zhu YS, Shen GF. Nicotiana chloroplast genome III. Chloroplast DNA evolution. Theor Appl Genet. 1982;61(1):73-9.
[12] Breiman A. Mitochondrial DNA diversity in the genera of Triticum and Aegilops revealed by southern blot hybridization. Theor Appl Genet. 1987;73(4):563-70.
[13] Komarnitskii IK, Kovtun EV, Gleba YuYu. Repeated DNA sequences in genus Nicotiana. Tsitol Genet. 1994; 28(6):20-7.
[14] Evans IJ, James AM, Barnes SR. Organization and evolution of repeated DNA sequences in closely related plant genomes. J Mol Biol. 1983;170(4):803-26.
[15] Molnar SJ, Gupta PK, Fedak G, Wheatcroft R. Ribosomal DNA repeat unit polymorphism in 25 Hordeum species. Theor Appl Genet. 1989;78(3):387-92.
[16] Santoni S, Bervill? A. Characterization of the nuclear ribosomal DNA units and phylogeny of Beta L. wild forms and cultivated beets. Theor Appl Genet. 1992;83(5):533-42.
[17] Borisjuk N, Borisjuk L, Petjuch G, Hemleben V. Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae. Genome. 1994;37(2):271-9.
[18] Komarnitskii IK, Komarnitskii SI. Size polymorphism of restricted fragments of rDNA intergenic spacer in some tobacco species. Tsitol Genet. 1996; 30(1):65-72.
[19] Komarnitskii IK, Komarnitskii SI. Variability of ribosomal DNA intergenic spacer in American tobacco lines. Tsitol Genet. 1997; 31(2):29-36.
[20] Borisjuk NV, Davidjuk YM, Kostishin SS, Miroshnichenco GP, Velasco R, Hemleben V, Volkov RA. Structural analysis of rDNA in the genus Nicotiana. Plant Mol Biol. 1997;35(5):655-60.
[21] Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97.
[22] Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980;8(19):4321-5.
[23] Reed KC, Mann DA. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985;13(20):7207-21.
[24] Church GM, Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984;81(7):1991-5.
[25] Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983;132(1):6-13.
[26] Kolosha VO, Fodor II. Structure heterogeneity of Citrus limon rDNA. Mol Biol (Mosk). 1986; 20(3): 656-62.
[27] Gerlach WL, Bedbrook JR. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res. 1979;7(7):1869-85.