Biopolym. Cell. 2001; 17(1):36-42.
Structure and Function of Biopolymers
Staphylococcus toxin modulates the regulatory mechanisms of contraction-relaxation of ring smooth muscles of the thick intestine
1Davidovskaya T. L., 1Tsimbalyuk O. V., 1Miroshnichenko M. S.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033


The influence of Staphylococcus toxin (ST) of concentration 1,6 · 10–6 –1,6 · 10–1 Lh on the contractile activity of thin (150–200 pm) smooth muscle stripes from guinea pig caecum caused by acetylcholine or high potassium solution and caffeine has been studied. It has been found that ST changes the permeability of L-type Ca channels dose-dependently and inhibits the caffeine-induced contraction of muscle stripes. ST modulates the realisation of a neurotransmitter signal along the electrical and pharma-comechanical ways of excitation-contraction coupling in smooth muscle cells.


[1] Krasil'nikov OV, Ternovski? VI, Tashmukhamedov BA. [Properties of conductivity channels induced in phospholipid bilayer membranes by alpha-staphylotoxin]. Biofizika. 1981;26(2):271-6.
[2] Cheferna I, Kadmek O, Machek K, Gulogpa O. Mechanisms of staphylococcal alpha-toxin action on guinea pig ureter in studies by GAP with sucrose. Zh Mikrobiol Epidemiol Immunobiol. 1974; 8(3): 270-4.
[3] Krasilnikov OV, Merzlyak PG, Yuldasheva LN, Rodrigues CG, Nogueira RA. Heparin influence on alpha-staphylotoxin formed channel. Biochim Biophys Acta. 1999;1417(1):167-82.
[4] Vornovitsky EG,, Ignatyeva VB, Pashutin SB, Belotsky SM. Effect of Staphylococcal toxin and its combination with antistaphylococcal gamma-globulin on electrical and contractile activity of the guinea-pig myocardium. Biull Eksp Biol Med. 1983; 96(10): 16-9.
[5] Brill' GE. [Effect of staphylococcal toxin on the calcium-transporting systems of the heart muscle]. Patol Fiziol Eksp Ter. 1985;(4):15-8.
[6] Bukharova TB, BeД­lbaeva ML, Ezepchuk IuV. [Interaction of staphylococcal alpha-toxin with biomembranes]. Mol Gen Mikrobiol Virusol. 1990;(8):3-4.
[7] Ohfsson A, Kaeus U, Hebert H. The structure of oligomeric a-toxin Staphylococcus aureus in human plateled membranes. EUREM 88: Proc. 9th Eur. Congr. Electron. Microsc. (New York, 4-9 Sept., 1988). Bristol, 1988: 397-8.
[8] Lala AK, Raja SM. Photolabeling of a pore-forming toxin with the hydrophobic probe 2-[3H]diazofluorene. Identification of membrane-inserted segments of Staphylococcus aureus alpha-toxin. J Biol Chem. 1995;270(19):11348-57.
[9] Brill' GE. [Mechanism of action of staphylococcal toxin on the heart]. Patol Fiziol Eksp Ter. 1988;(6):45-8.
[10] Rusakova EV. [Change in the hemolytic activity of staphylococcal delta- and alpha-toxins during interaction with organ tissues of experimental animals in vitro]. Zh Mikrobiol Epidemiol Immunobiol. 1973;50(8):81-5.
[11] Paster EU, Cheusova ZV, Vershigora AE. Staphylococcus toxins. Staphylococcus. Eds VV Smirnov, AE Vershigora. K.: Naukova Dumka, 1988: 14-34.
[12] Kosterin SA, Burdyga FV. [Kinetic analysis of contraction-relaxation process in smooth muscles]. Biofizika. 1990;35(2):350-5.
[13] Lakin GF. Biometrics. Vysshaya Shkola, 1990. 352 p.
[14] McDonald TF, Pelzer S, Trautwein W, Pelzer DJ. Regulation and modulation of calcium channels in cardiac, skeletal, and smooth muscle cells. Physiol Rev. 1994;74(2):365-507.
[15] Zholos OV. Membrane and intracellular mechanisms of M-cholinergic activation smoothly muscular cells of the small intestine: Thesis. ... Dr. biol. nauk. Kyiv, 1999. 278 p.
[16] Kochemasova NG, Shuba MF. Possible ways of extracellular calcium influx necessary for indication of contraction. Physiol, and Pharmacol, of Smooth Muscle: Abstrs. of papers. Varna: Publ. house Bulg. Acad. Sci., 1979: 44.
[17] Bolton TB, Lim SP. Properties of calcium stores and transient outward currents in single smooth muscle cells of rabbit intestine. J Physiol. 1989;409:385-401.
[18] Kuriyama H, Kitamura K, Itoh T, Inoue R. Physiological features of visceral smooth muscle cells, with special reference to receptors and ion channels. Physiol Rev. 1998;78(3):811-920.
[19] Somlyo AV, Bond M, Somlyo AP, Scarpa A. Inositol trisphosphate-induced calcium release and contraction in vascular smooth muscle. Proc Natl Acad Sci U S A. 1985;82(15):5231-5.
[20] Kurskiy MD, Kosterin SA, Rybalchenko VK. Biochemical kinetics. K.: Vyscha Shkola , 1977. 264 p.