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Short Communications
Formamide and glycerol in sequencing of GC-rich DNA
1, 2Kvasha S. M., 2, 3, 4Zabarovsky E. R., 1, 2, 3Kashuba V. I., 1Rynditch A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Center for Genomics and Bioinformatics,
    Karolinska Institutet
    SE-171 77 Stockholm, Sweden
  3. Department of Microbiology, Tumor and Cell Biology,
    Karolinska Institute
    Nobels vag 5 171 65 Solna, Sweden
  4. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
    32, Vavilova Str., Moscow, Russian Federation, 119991


Formamide and glycerol are known to increase the sequencing efficiency. We compared the ability of different concentration of these co-solvents to improve the sequencing results of GC-rich template. Herein, we report that addition of 5 % formamide and 7.5–10 % glycerol is the most effective for sequencing GC-rich templates.


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