Biopolym. Cell. 2003; 19(6):553-557.
Bioorganic Chemistry
Application of enzyme biosensor base on pH-sensitive field transistors for determination of glucose concentration in potato juice
1Skryshevska I. V., 1Korpan Y. I., 1Soldatkin A. P.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


A method for potato quality monitoring based on the determination of glucose concentration in potato juice using pH-sensitive field effect transistors with immobilized glucose oxidase has been proposed. Glucose concentration in potato juice was determined by a method of standard additions using the linear part of a calibration curve within dynamic range of 0.2—2 mM glucose concentration. To estimate the accuracy of glucose detection by the biosensor proposed, the measurements were also performed by a colorimetric method («Diagluc» enzymatic kit, Ukraine). A good correlation between these techniques is obtained.


[1] Pritchard M. Relationships of sugars to color of processed potatoes. Potato Facts Bull.-New York: Manitoba Agricult. and Food, publ., 1993: 4.
[2] Tai G, Xiong XY. Selection for low glucose content in potato breeding populations. Northeast Potato Technology Forum materials. Seabrook, 2001: 118.
[3] Sowokinos J. Choices for potatoes that chip directly from 42 F without reconditioning. Valley Potato Grower. 1999. 64, N 114: 14-17.
[4] Wu G. Disease resistance conferred by expression of a gene encoding H2O2-Generating glucose oxidase in transgenic potato plants. The Plant Cell. 1995;7(9):1357–68.
[5] Pat USA 5, 959, 180. DNA sequences from potato encoding solanidine UDP-glucose glucosyltransferase and use to reduce glycoalkaloids in solanaceous plants. Moehs C. P., Allen P., Rockhold E., Stapleton A., Friedman M., Belknap W.. Publ. 1999.
[6] Soldatkin AP, El’skaya AV, Shul’ga AA, Netchiporouk LI, Nyamsi Hendji AM, Jaffrezic-Renault N, et al. Glucose-sensitive field-effect transistor with additional Nafion membrane. Anal Chim Acta. 1993;283(2):695–701.
[7] Volotovsky VV, Patskovsky SV. Device for measuring of gate potential of ion-sensitive field effect transistor. Devices Exp. Tech. 1996; 3: 168.
[8] Soldatkin AP, Boubryak OA, Starodub NF, El’skaya AV, Sandrovsky AK, Shul’ga AA, Strikha VI. Urease biosensor based on field effect transistor. Design features and performance in a model conditions. Electrochemistry (Moskow). 1993; 29(3):315-9.
[9] Dzyadevich SV, Korpan YI, Arkhipova VN, Alesina MYu, Martelet C, El'Skaya AV, Soldatkin AP. Application of enzyme field-effect transistors for determination of glucose concentrations in blood serum. Biosens Bioelectron. 1999;14(3):283-7.
[10] Kim D, Goldberg I, Judy J. A micromashined amperometric nitrate sensor. Proc. Annu. Res. Rev. of UCLA Electrical Engineering Department. Los-Angeles, 2003: 19-25.
[11] Tretyakov NN, Koshkin EI, Makrushin NM. Physiology and biochemistry of agricultural plants. M.: Kolos, 2000; 640 p.
[12] Przybyt M. Influence of anions on glucose electrode response: application to extending concentration range. Biosens Bioelectron. 1998;13(3-4):471-7.