Biopolym. Cell. 1986; 2(1):23-29.
Structure and Function of Biopolymers
Investigation of polydisperse actin solutions by quasi-elastic light scattering
1Dobychin P. D., 1Lomakin A. V., 1Mevkh N. G., 1Noskin V. A., 1Balabonov S. M.
  1. B. P. Konstantinov Institute of Nuclear Physics, Academy of Sciences of the USSR
    Gatchina, Leningrad distr., USSR
  2. Institute of Biophysics, Academy of Sciences of the USSR
    Pushchino, USSR


The distribution function of actin in solution is restored from the quasi-elastic light scattering spectrum. The «micro-viscosity» of F-actin dilute solutions which is defined by mobility of latexes in the protein solution is discussed. Changes in the distribution function of protein are examined in the process of G-actin association and F-actin dissociation.


[1] Fujime S, Ishiwata S. Dynamic study of F-actin by quasielastic scattering of laser light. J Mol Biol. 1971;62(1):251-65.
[2] Carlson FD, Fraser AB. Dynamics of F-actin and F-actin complexes. J Mol Biol. 1974;89(2):273-81.
[3] Maeda T, Fujime S. Quasi-Elastic Light Scattering from Muscle F-Actin. J Phys Soc Jap. 1977. 42(6):1983-1991.
[4] Newman J, Carlson FD. Dynamic light-scattering evidence for the flexibility of native muscle thin filaments. Biophys J. 1980;29(1):37-47.
[5] Gulari E, Gulari E, Tsunashima Y, Chu B. Photon correlation spectroscopy of particle distribution. J Chem Phys. 1979; 70(8):3965-3972.
[6] Provencher S. Inverse problems in polymer characterization: Direct analysis of polydispersity with photon correlation spectroscopy. Macromol Chem. 1979; 180(1): 201-209.
[7] Braginskaya TG, Dobichin PD, Ivanova MA, Klyubin VV, Lomakin AV, Noskin VA, Shmelev GE, Tolpina SP. Analysis of the Polydispersity by Photon Correlation Spectroscopy. Regularization Procedure. Physica Scripta. 1983; 28(1):73-79.
[8] Spudich JA, Watt S. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J Biol Chem. 1971;246(15):4866-71.
[9] Klyubin VV, Noskin VA, Sakharova NA, Chechik OM. Study of the diffusive motion of spherical particles by optical mixing. Leningrad, (Preprint. AN SSSR. Leningrad, Inst. of Nuclear Physics; N 589). 1980; 25 p.
[10] Ivanova MA, Lomakin AV, Noskin VA. Dynamics of coiled DNA by the light scattering method. Mol Biol (Mosk). 1983;17(3):653-66.
[11] Koppel DE. Analysis of Macromolecular Polydispersity in Intensity Correlation Spectroscopy: The Method of Cumulants. J Chem Phys. 1972; 57(11):4814-20.
[12] Tobacman LS, Korn ED. The kinetics of actin nucleation and polymerization. J Biol Chem. 1983;258(5):3207-14.
[13] Wegner A, Engel J. Kinetics of the cooperative association of actin to actin filaments. Biophys Chem. 1975;3(3):215-25.
[14] Berne B. J., Pecora R. Dynamic light scattering with applications to chemistry, biology and physics. New York : J. Wiley and Sons, 1976. 376 p.
[15] Wegner A. Head to tail polymerization of actin. J Mol Biol. 1976;108(1):139-50.