Biopolym. Cell. 1986; 2(2):69-73.
http://dx.doi.org/10.7124/bc.00019E
Structure and Function of Biopolymers
Catalytic activity of aspartate aminotransferase microcrystals
1Kochkina V. M.
  1. Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

The enzymic activity of crystalline cytosolic aspartate aminotransferase (L-aspartate: aminotransferase 2-oxoglutarate, EC 2.6.1.1) was determined in suspensions of micro-crystals in 50 % ammonium sulphate and 20 % (wt/vol) polyethylene glycol. The crystals (1-2 μm wide) were small enough to preclude diffusional rate limitation. The catalytic activity of the crystalline enzyme is 32 % and 40 % of the enzyme activity in solution, respectively.
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