Biopolym. Cell. 1986; 2(4):200-206.
Cell Biology
Associative clone selection of chinese hamster cells for a complex of quantitative characters
1Savchenko V. K., 1Mikhalevich L. S., 1Kukushkina L. M.
  1. Institute of Genetics and Cytology, Academy of Sciences of the Byelorussian SSR
    Minsk, USSR


Associative clone selection for a complex of quantitative characters was carried out in populations of cultivated cells. Two hypotetraploid cell populations of Chinese hamster of plus- and minus-direction of selection were studied. The possibility of preserving relatively stable caryotypic structure of polyploid cell populations of spontaneous origin during 20 cell passages. An entropy index was used as a measure of caryotypic heterogeneity and general structure indefinitness of genetic systems – cell populations. A definite correlation between an increase in the genome number in a cell and a change in a set of quantitative characters was determined.


[1] Harris M. Mutation rates in cells at different ploidy levels. J Cell Physiol. 1971;78(2):177-84.
[2] Harris M. Polyploid series of mammalian cells. Exp Cell Res. 1971;66(2):329-36.
[3] McBurney MW, Whitmore GF. Selection for temperature-sensitive mutants of diploid and tetraploid mammalian cells. J Cell Physiol. 1974;83(1):69-74.
[4] Petrova ON, Manuilova ES, Shapiro NI. Hybridization of Chinese hamster cells sensitive to ultraviolet irradiation. Genetika. 1977;13(4):637-45.
[5] Morrow J, Stocco D, Barron E. Spontaneous mutation rate to thioguanine resistance is decreased in polyploid hamster cells. J Cell Physiol. 1978;96(1):81-6.
[6] Thompson KV, Holliday R. The longevity of diploid and polyploid human fibroblasts. Evidence against the somatic mutation theory of cellular ageing. Exp Cell Res. 1978;112(2):281-7.
[7] Brodskiy V, Uryvayeva IV. Cell polyploidy: Proliferation and differentiation. Moscow, Nauka, 1981; 260 p.
[8] Iashvili GD, Chernikov VG, Pichugina EM. Studying activity of nucleolar organizer regions of chromosomes in Chinese hamster clones different ploidy. Bull Exp Biol Med. 1983; 95(3):79-81.
[9] Savchenko VK. Associative selection and its role in the evolution and breeding. Zh Obshch Biol. 1980; 41(3):406-417.
[10] Savchenko VK. Genetic analysis in network test crosses. Minsk, Nauka i tekhnika, 1984; 224 p.
[11] Adams RLP. Cell Culture for Biochemists. Elsevier/North-Holland Biomedical Press. 1980; 292 p.
[12] Puck TT, Marcus PI. Action of X-rays on mammalian cells. J Exp Med. 1956;103(5):653-66.
[13] Moorhead PS, Nowell PC, Mellman WJ, Battips DM, Hungerford DA. Chromosome preparations of leucocytes cultured from human peripheral blood.Exp Cell Res. 1960;20:613-6.
[14] Venttsel' ES. Probability. Moscow, Nauka, 1969; 358 p.
[15] Vakhtin IuB, Ignatova TN, Fridlianskaia II, Shvemberger IN. Intensity of selection and rate of major karyotypic changes in the population of somatic cells during cloning. Tsitologiia. 1965;7(2):258-9.
[16] Bakhtin YB. Genetic theory of cell populations. Leningrad, Nauka, 1980; 168 p.
[17] Glebov OK, Abramian DS. Nature of the phenotypic variability of somatic cells in culture: unstable phenotypic changes. Tsitologiia. 1984;26(3):235-51.