Biopolym. Cell. 1986; 2(6):293-301.
Structure and Function of Biopolymers
High density lipoprotein conformation changes in the process of saturation by cholesterol
1Noskin V. A., 1Shmelev G. E., 1Lomakin A. V., 2Petrova-Mastakova L. G., 2Parfenova N. S., 2Belova E. V., 2Klimov A. N.
  1. B. P. Konstantinov Institute of Nuclear Physics, Academy of Sciences of the USSR
    Gatchina, Leningrad distr., USSR
  2. Institute of Experimental Medicine, Academy of Medical Sciences of the USSR
    Leningrad, USSR


By means of quasielastic light scattering the size of human high density lipoproteins (HDL3) has been determined depending on the time of their incubation with erythrocytes both in the presence of the lecitin-cholesterol-acyltransferase inhibitor (DTNB) and without it. It has been shown that in the presence of DTNB the exchange of cholesterol between HDL3 and membranes of erythrocytes is a first-order reaction. From the temperature dependence of rate constant the activation energy of cholesterol exchange Ea = 6.5 ± 0.2 kkal/mol has been calculated. The reaction rate appears to be proportional to membrane concentration, which evidences in favour of contact transfer of cholesterol. In the absence of DTNB the cholesterol exchange has a more complicated kinetics. An attempt has been made to explain the behaviour of HDL3 average size during incubation with erythrocytes both in the presence of DTNB and without it.


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