Biopolym. Cell. 1986; 2(6):311-316.
Structure and Function of Biopolymers
The all-machine synthesis of oligodeoxyribonucleotides by phosphotriester approach using automatic synthesizer «Victory-4M»
1Gryaznov S. M., 2Potapov V. K., 2Gorn V. V., 2Zarytova V. F., 2Sredin Yu. G., 3Potemkin G. A., 1Shabarova Z. A.
  1. M. V. Lomonosov State University
    Moscow, USSR
  2. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR
  3. Special Design Technological Office of the Special Electronics and Analytical Instrument Engineering, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR


The working regime of new model of automatic synthesizer of eligodeoxyribonucleotides «Victory-4M» applicable for two schemes of phosphotriester synthesis of oligonucleotides in 3'-5' direction has been developed, the nucleotide components being either dissolved in the solvents or immobilized on polymer supports. Both styrene-teflon copolymer and silochrome C-80 were used as polymer supports. The comparative analysis of the results of allmachine synthesis of the series of oligodeoxyribonucleotides has shown, that inspite of the differences in the structure and chemical nature of supports and nucleotide components, the average yield was about 75–80 % per step. These results demonstrated the efficiency of synthesizer and applicability of the both synthetic approaches for automatized synthesis of oligonucleotides.


[1] Potapov VK, Potemkin GA, Gorn VV, Zarytova VF, Sredin YuG, Shabarova ZA, Knorre DG. Semi-automatic solid-phase synthesis of oligodeoxyribonucleotides. Dokl Akad Nauk SSSR. 1982; 263(6):1386-9.
[2] Lomakin AI, Yastrebov SI, Popov SG. Automated synthesis of oligodeoxyribonucleotides. I. A study of solid supports based on silica gel "Silochrom". Russian Journal of Bioorganic Chemistry. 1985, 11 (7):920-926.
[3] Efimov VA, Reverdatto SV, Chakhmakhcheva OG. New effective method for the synthesis of oligonucleotides via phosphotriester intermediates. Nucleic Acids Res. 1982;10(21):6675-94.
[4] Rosenthal A, Cech D, Veiko VP, Orezkaja TS, Kuprijanova EA, Shabarova ZA. Triester-festphasensynthese von oligodesoxyribonucleotiden an polystyren-teflon trägern. Tetrahedron Lett. 1983; 26(16)1691-4.
[5] Gorn VV, Zarytova VF. Solid-phase synthesis of deoxyribooligonucleotides suitable for direct use in ligase reactions. Russian Journal of Bioorganic Chemistry. 1985; 11(6):808-14.
[6] Bausk EV, Gorn VV, Lebedev AV. General scheme of the solid-phase phosphotriester synthesis of 5'-phosphorylated-oligodeoxyribonucleotides on the basis of β-cyanoethyl p-chlorophenyl esters of N-acylnucleoside-5'-phosphates. Russian Journal of Bioorganic Chemistry. 1985; 11(6):815-20.
[7] Gryaznov SM. Testing of the regulations of the machine-oligonucleotide synthesizer "Victoria-3." MSU. Moscow, 1984; Dep. VINITI 30.08.84, N 6054.
[8] Gryaznova OI, Dolinnaya NG, Isagulyants MG, Metelev VG, Oretskaya TS, Udalov NI, Sokolova NI, Shabarova ZA. Studies of synthesis and thermal stability of oligodesoxyribonucleotide duplexes with structural anomalies. Russian Journal of Bioorganic Chemistry 1986, 12 (1):124-32.
[9] Jones SS, Reese CB, Sibanda S, Ubasawa A. The protection of uracil and guanine residues in oligonucleotides synthesis. Tetrahedron Lett. 1981; 22(47):4755-8.
[10] Dobrynin VN, Filippov SA, Byslrov NS, Severtsova IV, Kolosov MN. Methylimidazole catalyzed rapid phosphotriester synthesis of oligodeoxynucleotides on a silica gel support in dichloroethane. Russian Journal of Bioorganic Chemistry 1983, 9 (5):706-10.