Biopolym. Cell. 1986; 2(6):323-327.
Cell Biology
Interaction of alkylating oligonucleotide derivatives with mouse fibroblasts
1Vlassov V. V., 2Gorokhova O. E., 1Ivanova E. M., 1Kutyavin I. V., 1Yurchenko L. V., 1Yakubov L. A., 1, 2Abdukayumov M. N., 1Skoblov Yu. S.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR
  2. Experimental Unit, Institute of Nuclear Physics, Academy of Sciences of the Uzbek SSR
    Tashkent, USSR


Interaction of 32P labelled oligonucleotides and alkylating oligonucleotide derivatives carrying aromatic 2-chloroethylamine residues with mouse fibroblasts L929 has been investigated. At high concentrations of oligonucleotide derivatives in the culture medium, their uptake by the cells can be ascribed to pinocytosis. At low concentrations (less than 2μM) the efficiency of the uptake is much higher, in particular in the case of the derivatives carrying 2-chloroethylamino groups, most probably due to their absorption on the cells. The binding kinetics plateaus are given 2 hours after incubation at 37 °C. Alkylating oligonucleotide derivatives taken up by the cells efficiency modify cellular biopolymers and remain undegraded in cells 3 hours after incubation at 37 °C. The derivatives show no cytotoxic action at the concentrations used.


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