Biopolym. Cell. 1986; 2(6):328-334.
Viruses and Cell
The discovery and study of subgenomic RNA of potato virus X
1Grama D. P., 2Mashkovsky N. N.
  1. Institute of Microbiology and Virology, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Virus-specific, low-molecular weight RNA (m. m. 0.3·106) is detected in Datura plants infected by potato virus X, using 32P-cDNA synthesized on PVX RNA. The low-molecular weight RNA is observed in infected plants three days after infection and it sediments in the linear sucrose gradient between 18S and 5S by ribosomal RNAs, has a nucleotide sequence to 3'-end of genome RNA. It translates in cell-free system into protein with electrophoretic mobility identical to that of coat protein, i. e. it is subgenomic PVX RNA for synthesis of virus coat protein.


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