Biopolym. Cell. 2005; 21(4):312-318.
Cell Biology
Paenibacillus sp., as a promising candidate for development of a novel technology of inoculant production
1Kozyrovska N. O., 1Negrutska V. V., 1Kovalchuk M. V., 1Voznyuk T. N.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


A bacterial strain IMBG156 producing exopolysaccharide (EPS) was isolated from siliceous rock and identified as a Paenibacillus species by partial sequencing its 16S rDNA. Paenibacillus sp. IMBG156 was used in a novel technology for inoculant production based on co-cultivating this bacterium with any bacterium of choice. Paenibacillus sp. provides in situ the bacterial cells of the inoculant with EPS, a carrier, and most likely with a source of carbon and energy. The partner bacterium designates a type of inoculant (biopesticide or biofertiliser). The strain IMBG156 does not destroy the signaling system of Gram-negative partners, based on acylated homoserine lactones, stimulates plant growth, and is rather competitive in the plant rhizosphere and soil. A prototype of the inoculant based on dual-culture Paenibacillus sp. IMBG156 - Pseudomonas sp. IMBG163 exhibits a noticeably longer shelf life than monoculture of Pseudomonas sp. IMBG163.
Keywords: exopolysaccharide, inoculant, partner bacteria


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