Biopolym. Cell. 2005; 21(4):335-345.
Cell Biology
The concept of genetic material exchange between mammalian cells
1Kordyum V. A., 1Shpilevaya S. P., 1Ruban T. O., 1Sukhorada O. M.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The concept presented is confirmed by both direct experiments on the genetic markers transfer and microscopic observations. The authors formulate an idea that there is a universal informational space of the organism, created due to the DNA release within the lifetime of cells without destroying their genomes (and DNA absorption, complementing the mutations) or due to the addressed transfer of genetic material by specialized cells.
Keywords: genetic information transfer, cells interaction


[1] Kordyum VA. Our "Shagreen leather" is our problem. And we have to solve it. 3. A missing link. Biopolym Cell. 2003; 19(6):473-91.
[2] Kordyum V, Suhorada O, Ruban T, Shpilevaya S, Andrienko V, Deryabina O. Transfer of genetic information in an organism. First Ukr Congr Cell Biol. Lvlv, 2004:146.
[3] Shpilevaya SP, Andrienko VI, Sukhorada OM, Ruban TA, Deryabina OC, Likhacheva LL, Irodov DM, Kordyum VA. Interaction of mouse fetal liver cells with CHO-K1 cells in vitro accompanying with genetic information transfer. Joint Meeting "Tissue Engineering Society International* and В«European Tissue Engineering Society": Abstract book. Lausanne, 2004: 229.
[4] Shpilevaya SP, Andrienko VI, Ruban TA, Sukhorada EM, Irodov DM, Kordyum VA. Interaction of mouse fetal liver cells with CHO-1 cells. Faktori eksperimental'noi evolucii organizmiv, 2004;2:83-7.
[5] Suhorada O, Ruban T, Deryabina O, Toporova O, Kordyum V. Transformation of mammalian cells during interaction of two partners consisting of different cells: Abstracts XXX Annu. ESAO Congr Int J Artificial Organs. 2004; 608.
[6] Kordium VA, Shpilevaya SP, Ruban TA, Sukhorada OM, Andriyenko VI. Autotransformation of mammalian cells. Biopolym Cell. 2005; 21(2):140-4.
[7] Kordyum VA, Shpylova SP, Andrienko VI, Sukhorada OM, Ruban TA, Deryabina OG. Transfer of genetic information in an organism. Cell Biol Int. 2005;29(1):95-7.
[8] Kordyum VA, Toporova EK, Okunev OV, Pokholenko YA, Suchorada EM, Ruban TA, Andrienko VI, Irodov DM. Novel cell line with multiple markers, derivative of CHO-K1. Biopolym Cell. 2003; 19(3):252-6.
[9] Pokholenko YA, Suchorada EM, Ruban TA, Toporova OK, Okunev OV, Andrienko VI, Kordyum VA. Creation of transgenic mammals sublines with multiple selection markers . Faktori eksperimental'noi evolucii organizmiv, 2003:399-404.
[10] Baraldi PG, Bovero A, Fruttarolo F, Preti D, Tabrizi MA, Pavani MG, Romagnoli R. DNA minor groove binders as potential antitumor and antimicrobial agents. Med Res Rev. 2004;24(4):475-528.
[11] Loken MR. Separation of viable T and B lymphocytes using a cytochemical stain, Hoechst 33342. J Histochem Cytochem. 1980;28(1):36-9.
[12] Loken MR. Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter. Cytometry. 1980; 1(2): 136-142.
[13] Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med. 1996;183(4):1797-806.
[14] Berardi AC, Wang A, Levine JD, Lopez P, Scadden DT. Functional isolation and characterization of human hematopoietic stem cells. Science. 1995;267(5194):104-8.
[15] Animal Cell Culture. Methods. M.: Mir, 1989. 332 p.
[16] Shimizu N, Itoh N, Utiyama H, Wahl GM. Selective entrapment of extrachromosomally amplified DNA by nuclear budding and micronucleation during S phase. J Cell Biol. 1998;140(6):1307-20.
[17] Sait SN, Qadir MU, Conroy JM, Matsui S, Nowak NJ, Baer MR. Double minute chromosomes in acute myeloid leukemia and myelodysplastic syndrome: identification of new amplification regions by fluorescence in situ hybridization and spectral karyotyping. Genes Chromosomes Cancer. 2002;34(1):42-7.
[18] Stark GR. Regulation and mechanisms of mammalian gene amplification. Adv Cancer Res. 1993;61:87-113.
[19] Kurchatova SYu, Kireev II, Polyakov IYu. Investigation of the local reorganization of the nuclear envelope, revealed at hypotonic treatment of living cells in S-phase of the cell cycle. microscopic studies. M.: Izd MGU, 2004;20-30.
[20] Gall JG, Rochaix JD. The amplified ribosomal DNA of dytiscid beetles. Proc Natl Acad Sci U S A. 1974;71(5):1819-23.
[21] Kopnin BP, Gudkov AV. [Amplification of genome regions in the somatic cells of mammals resistant to colchicine. III. Localization of the amplified genes in minute chromatin bodies]. Genetika. 1982;18(10):1683-92.
[22] Lillie RD. Histopathologic technic and practical histochemistry. Blakiston Division, McGraw-Hill, 1965; 715 p
[23] Lalande ME, Miller RG. Fluorescence flow analysis of lymphocyte activation using Hoechst 33342 dye. J Histochem Cytochem. 1979;27(1):394-7.