Biopolym. Cell. 2005; 21(4):365-369.
Preparation of bifunctional silica polymer support for the synthesis of 3'-labeled oligonucleotides
1Dubey L. V., 1Dubey I. Ya.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Preparation of a silica polymer support with a linker arm containing protected amino and hydroxy groups is described. O-Benzotriazol-1-yl-N,N,N'‚N'-tetramethyluronium hexafluorophosphate (HBTU) in the presence of 1-hydroxybenzotriazole (HOBT) was used as an efficient activating reagent at several steps of the polymer synthesis. This bifunctional support is suitable for the preparation of 3'-amino-modified oligonucleotides for post-synthetic conjugation, as well as for the direct solid phase synthesis of 3'-labeled oligonucleotides. The fluorescein-modified silica polymer was synthesized.
Keywords: polymer supports, onium reagents, labeled oligonucleotides


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