Biopolym. Cell. 2006; 22(3):186-191.
Structure and Function of Biopolymers
Removal of part of the eEF1A GTP binding domain induced translation errors in vitro
1Pogribna A. P., 1Negrutskii B. S., 1Elskaya A. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Eukaryotic translation elongation factor 1A (eEF1A) is one of the main components of the translation machinery acting at the elongation step. Oncogene PTI-1 which is expressed during prostate cancer development, codes the protein, homologous to eEF1A. In this paper the hypothesis is tested that the gene PTI-1 protein product can influence accuracy of translation. The ability of the PTI-1 protein analogue to stimulate misincorporation of leucine by ribosomes programmed with poly(U) was demonstrated. This finding favors the hypothesis about the ability of the PTI-1 oncogene product to influence translation accuracy in vivo.
Keywords: eukaryotic translation elongation factor 1A, oncogene PTI-1, ribosome, tRNA


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