Biopolym. Cell. 2006; 22(3):243-245.
Short Communications
Cultivation, purification and crystallization of virus of green algae Tetraselmis viridis
1Sizov D. V., 1Polischuk V. P.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01033


The method of obtaining the virus of algae Tetraselmis viridis in high concentration is presented and the scheme of its crystallization is developed which will enable henceforth the determination of virion structure on condition that the crystals correspondence to the requirements of the X-ray structural analysis. The obtained data are also useful for the elaboration of a new model system of cultivating viruses, in particular, phycodnaviruses.
Keywords: virus Tetraselmis viridis, virion structure, cultivation of viruses, phycodnaviruses


[1] Brussard CPD. Role of viruses in controlling phytoplankton blooms. Ecology of marine viruses (Banyuls-sur-mer, 19—22 March 2003). Monaco, 2003:67—73.
[2] Castberg T. Algal viruses: Characteristics and ecological ef­ fects.—DS. Thesis: Univ. of Bergen, 2001; 204 p.
[3] Stepanova OA. Algo viruses of the bays of Sevastopol. Botanical research in Asian Russia: Proceedings of the XI Congress of Russian Botanical Society (18-22 Aug 2003). Barnaul: Izd AzBuka, 2003; vol 1;143-4.
[4] Pat. 65864A Ukraine, MKI 7 C12 N1/12. N 2003065499. Isolation method algo viruses unicellular algae, for example Platymonas viridis Roach (Chlorophyta). OA Stepanova. Promyslova vlasnist. 2004;4:1.
[5] Cultivation of collection strains of algae. Mezhvuz. sb Ed BV Gromov. L, 1983. 153 p.
[6] McPherson R. Protein crystallization. Hampton, 2004; 255 p.
[7] Stepanova OA, Boiko AL, Gordienko AI, Sherban' SA, Shevchenko TP, Polishchuk VP. The characreristic of virus Tetraselmis viridis Norris (Chlorophyta Prasinophycea). Dopovidi Nats Akad Nauk Ukrainy. 2005; (1):158-62