Biopolym. Cell. 2006; 22(6):425-432.
Structure and Function of Biopolymers
Isolation and purification isoaccepting tRNA1Ser and tRNA2Ser from Thermus thermophilus
1Krikliviy I. A., 1Kovalenko O. P., 1Gudzera O. Y., 1Yaremchuk A. D., 1Tukalo M. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Serine aminoacylation system is unique as it is the only system where synthetase of class II recognizes tRNA with a long variable stem (type II tRNA) which is of great interest to study the mechanisms of tRNA recognition by corresponding aminoacyl-tRNA synthetase. Current paper presents the methodological approaches for obtaining purified isoacceptor tRNA1Ser and tRNA2Ser in milli­gram quantities from extremal thermophil T. thermophilus for crystallography. The developed method of tRNA purification employs the chromatography on benzoylated diethylaminoethylcellulose and high-performance liquid chromatography on anionexchange column Spherogel TSK DEAE 5PW, and reverse phase column Ultrapore C8 using HPLC equipment (Beckman).
Keywords: tRNA, chromatography, high-performance liquid chro­ matography, extremal thermophil Thermus thermophilus, benzoyla­ ted diethylaminoethylcellulose.


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