Biopolym. Cell. 2006; 22(6):439-445.
Molecular and Cell Biotechnologies
The distribution of injected plasmid DNA throughout the organs and the expression of human apoA-1 gene in vivo
1Gilchuk Yu. M., 1Irodov D. M., 1Starokadomskyy P. L., 2Pishel I. M., 1Toporova O. K., 2Novikova S. M., 1Kordium V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Gerontology Institute of the Academy of Medical Sciences of Ukraine
    67, Vyshgorodska Str., Kyiv, Ukraine, 04114


The elaboration of methods of gene delivery into somatic cells is the foundation of gene therapy development. There is necessity to develop new and more effective vectors as well as corresponding methods of gene delivery into the target tissues. The analysis of distribution of vector molecules in tissues and transgene expression are the constituent parts of evaluation of effectiveness of gene therapy methods for a target gene delivery. The study on the transfer of the plasmid DNA, containing human apoA-1 gene, in complex with polyethylenimine (PEI) into rat and rabbit liver parenchyma has been performed. The expression of human apoA-1 gene in vivo has been examined
Keywords: apolipoprotein A-l, polyethylenimine, transgene exp­ression, transgene distribution


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