Biopolym. Cell. 2006; 22(6):446-451.
Molecular and Cell Biotechnologies
Artificial skin equivalent based on copolymeric hydrogel membranes with immobilized human mesenchymal stem cells
1Kosenko O. O., 2Lukash L. L., 1Samchenko Yu. M., 2Ruban T. A., 1Lukash S. I., 1Ulberg Z. R., 3Galagan N. P.
  1. Ovcharenko Institute for Biocolloidal Chemistry, NAS of Ukraine
    42, Vernadsky blvd., Kyiv, Ukraine, 03142
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  3. Institute of Surface Chemistry, NAS of Ukraine
    17, Generala Naumova Str., Kyiv, Ukraine, 03164


The basic parameters of hydrogel membranes, which are important for their characteristics as potential biocompatible non-degradable wound coverings, have been optimized. Moderately hydrophobic and at the same time moderately hydrophilic membranes obtained on the basis of copolymerization of acrylamide and acrylonitrile were demonstrated to be biocompatible with mesenchymal stem cells. Small concentrations of highly dispersed silica introduced into a composition of hydrogel membranes were demonstrated to influence the cell cultivation positively. The technological conditions necessary for more effective and prolonged cultivation of cells on the surface of hydrogel membranes with improved exploitation parameters have been established
Keywords: skin equivalent, hydrogel, mesenchymal stem cells, immobilization, cultivation, nutrient medium


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