Biopolym. Cell. 2008; 24(2):171-175.
Bioorganic Chemistry
Synthesis of fluorescently labeled oligonucleotide conjugate with transport peptide on modified Silochrom-2 support
1Dubey I. Ya.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Synthesis of triple conjugate of oligonucleotide with transport peptide and fluorophore is described. 5'-aminoalkyl-modified 3'-fluorescein-labeled 18-mer oligonucleotide, complementary to the region of E. coli β-galactosidase gene was prepared by solid phase synthesis on fluorescein-modified support Silochrom-2. Its treatment with iodoacetic anhydride led to the formation of iodoacetamide derivative. The latter reacted specifically with cystein-containing 10-mer peptide, a fragment of the transport domain of large T-antigen of SV40 virus, to provide high yield of target hybrid.
Keywords: oligonucleotide conjugates, transport peptides, fluorescein


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