Biopolym. Cell. 2008; 24(4):300-309.
Molecular and Cell Biotechnologies
Rauwolfia serpentina Benth. cell populations dynamics in the context of different cultivation conditions in vitro
1, 2Parnikoza I. Yu., 2Miriuta N. Yu., 2Al-Ammouri Yu., 2Kunakh V. A.
  1. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01601
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The article describes a dynamics of the cell populations of R. serpentina strain K-27, which is a superproducent of anti-arrhythmic alkaloid aimaline, depending on the sucrose and inorganic substances contents and the tissue position in the culture (superficial or submerge). The authors have analysed the dynamics of cell quantity judging from the relative DNA content and nucleolus area. The specific rate of productivity, namely the biomass and indoline alkaloids accumulation, have been established in dependence on dynamics of definite cell subpopulations. The influence of all cultivation versions on cell populations variation in both cell parameters have been studied. Thermodynamic approach have been used to describe specific accumulation changes rate of biomass, tracheids and alkaloids that depend on the cell subpopulations dynamics were analyzed by paired linear regression.
Keywords: plant tissue culture, Rauwolfia serpentina, cell population dynamicsin vitro


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