Biopolym. Cell. 2009; 25(1):73-76.
Short Communications
Investigation of HSP60 gene expression in mRNA level in heart at dilated cardiomyopathy
1Kapustian L. M., 1Rozhko O. T., 1Bobyk V. I., 2Riabenko D. V., 1Sidorik L. L.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Scientific Center "M. D. Strazhesko Institute of Cardiology, MAS of Ukraine"
    5, Narodnogo Opolchennya Str., Kyiv, Ukraine, 03151


The expression of HSP60 in the mRNA level in human hearts at the end-stage of dilated cardiomyopathy (DCM) as well as in the hearts of mice with disease model similar to human DCM was investigated. We observed a significant increase in the Hsp60 mRNA level at the beginning of the disease and decrease to a normal level at the end stage. As the Hsp60 level was increased during the disease up to the end stage we can presume some changes in the regulation of Hsp60 synthesis or its degradation at DCM progression.
Keywords: molecular chaperones, Hsp60, dilated cardiomyopathy, mRNA


[1] Reeve J. L. V., Duffy A. M., O'Brien T., Samali A. Don't loose heart – therapeutic value of apoptosis prevention in the treatment of cardiovascular disease J. Cell. Mol. Med 2005 9, N 3:609–622.
[2] Gupta S., KnowltonA. A. Hsp60, Bax, apoptosis and the heart J. Cell. Mol. Med 2005 9, N 1:51–58.
[3] Kirchhoff S. R., Gupta S., Knowlton A. A. Cytosolic heat shock protein 60, apoptosis, and myocardial injury Circulation 2002 105:2899–2904.
[4] Shan Y. X., Liu T. J., Su H. F., Samsamshariat A., Mestril R., Wang P. H. Hsp10 and Hsp60 modulate Bcl-2 family and mitochondria apoptosis signaling induced by doxorubicin in cardiac muscle cells J. Mol. Cell. Cardiol 2003 35 P. 1135–1143.
[5] Kapustian L. M., Rozhko O. T., Bobyk V. I., Kroupska I. V., Ryabenko D. V., Khozhaenko Yu. S., Gurtovyy V. A., Usenko V. S., Sidorik L. L. Changes in the content of molecular chaperone Hsp60 in heart tissue at dilated cardiomyopathy Biopolym. Cell 2008 24, N 3:238–245.
[6] Bobyk V. I., Ryabenko D. V., Sergienko O. V., Trunina I. V., Fedorkova O. M., Morozova L. M., Sidorik L. L. Experimental model of autoimmune myosin-induced myocardium injury Biopolym. Cell 2007 23, N 2 P.115–122.
[7] Ryabenko D. V., Sidorik L. L., Bobyk V. I., Sergienko O. V., Fedorkova O. M., Trunina I. V., Matsuka G. Kh. Morphological features of autoimmune damage of myocardium caused by various human myocardial antigens: comparative experimental examination. Ukr. J. Rheumatol. 2000; N 2:55–60.
[8] Latif N., Taylor P. M., Khan M. A., Yakoub M. H., Dunn M. J. The expression of heat shock protein 60 in patients with dilated cardiomyopathy Basic Res. Cardiol 1999 94 P. 112–119.