Biopolym. Cell. 2009; 25(4):315-318.
Short Communications
Study on toxin-neutralization properties of recombinant single-chain variable antibody’s fragments against diphtheria toxin B subunit
1Oliinyk O. S., 1Labyntsev A. J., 1Korotkevich N. V., 1Kolibo D. V., 1Komisarenko S. V.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601


Aim. To select scFv which have an ability to neutralize diphtheria toxin. Methods. Flow-cytometry was used to study the ability of specific scFv to block the binding of a labeled receptor binding fragment of toxin with a surface of sensitive to toxin cells. Results. Four scFv with toxin-neutralization properties were selected. Conclusions. The obtained scFv could be considered as promising candidates for antitoxic antidiphtheria drugs of new generation.
Keywords: antitoxins, scFv, diphtheria toxin


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