Biopolym. Cell. 2009; 25(6):509-512.
Short Communications
Expression of nicotinic acetylcholine receptors on human B-lymphoma cells
1Omelchenko D. M., 1Skok M. V.
  1. Palladin Institute of Biochemistry, NAS of Ukraine
    9, Leontovycha Str., Kyiv, Ukraine, 01601


Aim. To find a correlation between the level of nicotinic acetylcholine receptor (nAChR) expression and B lymphocyte differentiation or activation state. Methods. Expression of nAChRs in the REH, Ramos and Daudi cell lines was studied by flow cytometry using nAChR subunit-specific antibodies; cell proliferation was studied by MTT test. Results. It is shown that the level of α4β2β4 and α7 nAChRs expression increased along with B lymphocyte differentiation (Ramos > REH) and activation (Daudi > Ramos) and depended on the antigen-specific receptor expression. The nAChR stimulation/blockade did not influence the intensity of cell proliferation.
Keywords: nicotinic acetylcholine receptor, B- lymphocytes


[1] Paterson D., Nordberg A. Neuronal nicotinic receptors in the human brain Prog. Neurobiol 2000 61, N 1:75–111.
[2] Conti-Fine B. M., Navaneetham D., Lei S., Maus A. D. Neuronal nicotinic receptors in non-neuronal cells: new mediators of tobacco toxicity? Eur. J. Pharmacol 2000 393, N 1–3:279–294.
[3] Grando S. A. Cholinergic control of epidermal cohesion in norm and pathology Exp. Dermatol 2006 15, N 4 P. 265–282.
[4] Kawashima K., Fujii T. Expression of non-neuronal acetylcholine in lymphocytes and its contribution to the regulation of immune function Front. Biosci 2004 9:2063– 2085.
[5] Wang H., Yu M., Ochani M., Amella C. A., Tanovic M., Susarla S., Li J. H., Yang H., Ulloa L., Al-Abed Y., Czura C. J., Tracey K. J. Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation Nature 2003 421, N 6921:384–388.
[6] Skok M., Grailhe R., Agenes F., Changeux J. P. The role of nicotinic acetylcholine receptors in lymphocyte development J. Neuroimmunol 2006 171, N 1–2:86–98.
[7] Skok M. V., Grailhe R., Agenes F., Changeux J. P. The role of nicotinic receptors in B-lymphocyte development and activation Life Sci 2007 80, N 24–25:2334–2336.
[8] Skok M. V., Kalashnik E. N., Koval L. N., Tsetlin V. I., Utkin Y. N., Changeux J. P., Grailhe R. Functional nicotinic acetylcholine receptors are expressed in B lymphocyte-derived cell lines Mol. Pharmacol 2003 64, N 4:885–889.
[9] Skok M. V., Voitenko L. P., Voitenko S. V., Lykhmus E. Y., Kalashnik E. N., Litvin T. I., Tzartos S. J., Skok V. I. Alpha subunit composition of nicotinic acetylcholine receptors in the rat autonomic ganglia neurons as determined with subunitspecific anti-alpha(181–192) peptide antibodies Neuroscience 1999 93, N 4:1427–1436.
[10] Koval O. M., Voitenko L. P., Skok M. V., Lykhmus E. Y., Tsetlin V. I., Zhmak M. N., Skok V. I. The b-subunit composition of nicotinic acetylcholine receptors in the neurons of the guinea pig inferior mesenteric ganglion Neurosci. Lett 2004 365, N 2:143–146.
[11] Carmichael J., DeGraff W. G., Gazdar A. F., Minna J. D., Mitchell J. B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity. Cancer Res. 1987; 47(4):943–946.
[12] Koval L. M., Lykhmus O. Yu., Omelchenko D. M., Komisarenko S. V., Skok M. V. The role of alpha7 nicotinic acetylcholine receptors in B lymphocyte activation Ukr. Biokhim. Zh. 2009; 81(4):5–11.
[13] Buerstedde J. M., Reynaud C. A., Humphries E. H., Olson W., Ewert D. L., Weill J. C. Light chain gene conversion continues at high rate in an ALV-induced cell line EMBO J 1990 9, N 3:921–927.
[14] Omelchenko D. M., Kalashnik O. M., Koval L. M., Komisarenko S. V., Skok M. V. Analysis of signaling pathways activated by nicotinic acetylcholine receptors in b-lymphocytederived cells. Ukr. Biokhim. Zh. 2009; 81(1):59–66.