Biopolym. Cell. 2013; 29(3):215-220.
Novel epigenetic markers of early epithelial tumor growth and prognosis
1Gordiyuk V. V., 1Kondratov A. G., 1Gerashchenko G. V., 1Kashuba V. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


The present work is aimed at clarifying genetic and epigenetic alterations that occur during carcinogenesis and designing perspective sets of newly identified biomarkers. The tumors of kidney, cervix, colon, ovary, and lung were analyzed in our work, using the chromosome 3 specific NotI microarrays (NMA). We have found loci/genes with essential changes in gene methylation of tumor samples. Changes in expression for these genes were confirmed. The Not-I microarray results have been used to develop epigenetic marker panels for the early detection of different tumor types (ovary and lung cancer), to discriminate the stages of tumor growth and to determine whether the tumor is metastasizing. Marker panel designing is of great perspective in clinical medicine.
Keywords: epithelial tumor, epigenetic markers, NotI-microarrays, early cancer detection, prognosis of cancer, tumor-suppressor gene


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