Biopolym. Cell. 1987; 3(2):59-65.
Structure and Function of Biopolymers
Localization of antigenic determinants of beef tryptophanyl-tRNA synthetase revealed by monoclonal antibodies
1Beresten S. F., 1Rubikaite B. I., 1Kazakov V. K.
  1. Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR


The method of limited cleavage of tryptophanyl-tRNA synthetase with trypsin and BrCN as well as radioimmunoassay and radioimmunoblotting are used to show that the antigenic determinant for monoclonal antibodies Ami is located within the 5 kDa fragment and for the Am3 – within the 9 kDa fragment, the both are located at the N-termini of the polypeptide chains; the antigenic determinant for Am2 residues is within the 24 kDa fragment located in the middle of tryptophanyl-tRNA synthetase polypeptide chain. BrCN-fragment maintaining the Ami antigenic determinant is purified by HPLC; it contains 22–25 amino acid residues. Tryptic fragments and fragments obtained by cleavage only at arginine residues are immunologically inactive against Ami, Am2 and Am3.


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