Biopolym. Cell. 1987; 3(2):83-87, 100.
Cell Biology
Behaviour of centrosome in the spreading of the transformed fibroblasts
1Gudima G. O., 1Vorobjev I. A., 1Chentsov Yu. S.
  1. M. V. Lomonosov State University
    Moscow, USSR


In L-strain fibroblasts at all stages of spreading (primary attachment, radial spreading, polarization, monolayer) centrioles are randomly oriented to the substrate plane. In polarized cells centrioles are located between the nucleus and the leading edge. In suspended cells short microtubules appear near the centrioles in a number exceeding that in monolayer culture cells. The outgrowth of microtubules from the centrosome is observed in radially spread and polarized L-cells. In contrast to normal murine fibroblasts the number of long and middle microtubules radiating from the active centrioles does not differ significantly in the radially spread, polarized and monolayer culture L-cells. This fact confirms the suggestion that the orientation of centrioles perpendicular to the substrate plane which is observed during the spreading and polarization of fibroblasts is connected with the intensified growth of microtubules from the centrosome. On the basis of a comparative analysis of the centrosome behaviour in normal, slightly transformed (3T3) and strongly transformed (L) fibroblasts the orientation of the active cenlrioles perpendicular to the substrate plane and the activation of microtubule formation in the centrosome during spreading and polarization is supposed to be dependent on a degree of morphological transformation of fibroblasts.


[1] Gudima GO, Vorob'ev IA, Chentsov IuS. Behavior of the cell center in the distribution of fibroblasts. Nauchnye Doki Vyss Shkoly Biol Nauki. 1983;(7):45-50.
[2] Gudima GO, Vorob'ev IA, Chentsov IuS. Structural change in the cell center during fibroblast polarization and movement. Tsitologiia. 1983;25(8):883-8.
[3] Zatsepina OV, Polyakov VYu, Chentsov YuS. Some structural aspects of the fate of the nuclear envelope during mitosis. Cytobiologie. 1977; 16(1):130-44.
[4] Rovenskii IuA, Ol'shevskaia LV. Dynamics of the process of spreading of normal fibroblast-like cells and transformed line L fibroblasts on the surface of a smooth substrate. Study using the scanning electron microscope Tsitologiia. 1973;15(8):1029-36.
[5] Albrecht-Buehler G, Bushnell A. The orientation of centrioles in migrating 3T3 cells. Exp Cell Res. 1979;120(1):111-8.
[6] Gotlieb AI, Heggeness MH, Ash JF, Singer SJ. Mechanochemical proteins, cell motility and cell-cell contacts: the localization of mechanochemical proteins inside cultured cells at the edge of an in vitro "wound". J Cell Physiol. 1979;100(3):563-78.
[7] Kupfer A, Louvard D, Singer SJ. Polarization of the Golgi apparatus and the microtubule-organizing center in cultured fibroblasts at the edge of an experimental wound. Proc Natl Acad Sci U S A. 1982;79(8):2603-7.
[8] Vorob'ev IA, Chentsov IuS. Centrioles in the cellular cycle. II. The interphase and 1st half of mitosis. Nauchnye Doki Vyss Shkoly Biol Nauki. 1978;(11):54-64.
[9] Gudima GO, Vorob'ev IA, Chentsov IuS. Behavior of the cell center during epithelial cell spreading. Tsitologiia. 1986;28(6):576-81.
[10] Tucker RW. Role of microtubules and centrioles in growth regulation of mammalian cells. Cell Muscle Motil. 1983;3:259-95.