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Gene-Engineering Biotechnology
Effect of lysogenization on the symbiotic properties of nodule bacteria of lucerne
1Novikova N. I., 1Simarov B. V.
  1. All-Union Research Institute of Agricultural Microbiology
    Leningrad, USSR


Twelve lysogenic clones were isolated from each mutants (M5, M31 and Dl) of nonlysogenic strain Rhizobium meliloti L5-30 while using three bacteriophages (Rml, Rm2 and Rm3). These lysogenic strains were resistant to their own phages and released them spontaneously and after UV-induction which resulted in an increase of the phage titre by 1–2 order. Electrophoretic analysis did not reveal change in plasmid contents. It was shown that lysogenization of R. meliloti strains can decrease their nitrogen-fixing activity and efficiency as compared with initial nonlysogenic cultures.


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