Biopolym. Cell. 1987; 3(3):160-163.
Short Communications
Factor-free poly (dT)-dependent synthesis of oligophenylalanine on Escherichia coli 70S ribosomes
1Soldatkin K. A., 1Potapov A. P., 1Elskaya A. V.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


The activity of poly(U) and poly(dT) in the factor-free binding of Phe-tRNAPhe to the E. coli 70S ribosomes has been compared to study the role of the codon sugar-phosphate backbone in the mRNA decoding process. Poly(dT) is shown to be effectively translated in such a factor-free system at both low (2 °C) and high (37 °C) temperatures. Under the same conditions poly(U) is not translated. Neomycin inhibits factor-free poly(dT) translation and has no effect on the binding of Phe-tRNAphe to poly(U) programmed ribosomes. The data obtained indicate the great importance of the template and its sugar-phosphate backbone in the translocation process.


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