Biopolym. Cell. 2014; 30(4):273-278.
The interaction of polyhydroxyalkanoate polymer group with cells and tissues
1Maiborodin I. V., 1Kuznetsova I. V., 1Shevela A. I., 1Manaev A. A., 1Chastikin G. A.
  1. Center of New Medical Technologies
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the RAS
    8, Akademika Lavrentieva Ave., Novosibirsk, Russian Federation, 630090


In the recent literature devoted to the results of the study, formulation and application of polyhydroxyalkanoates (PHA), contains a wealth of experimental and clinical data on efficacy and safety of using these polymers with biomedical goals. However, the application of PHA, both independently and in combination with other drugs and substances that must surely have its advantages and disadvantages. It should be noted the complete absence, information on contraindications and complications of the PHA, and insufficient data on the patterns of degradation of PHA in vivo, and morphological processes connected with it of scientific literature.
Keywords: polyhydroxyalkanoates, reconstructive medicine, biodegradation, cellular technologies


[1] Vacanti CA, Vacanti JP. The science of tissue engineering. Orthop Clin North Am. 2000;31(3):351-6.
[2] Fu X, Li H. Mesenchymal stem cells and skin wound repair and regeneration: possibilities and questions. Cell Tissue Res. 2009;335(2):317-21.
[3] Caplan AI. Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J Cell Physiol. 2007;213(2):341-7.
[4] Mansilla E, Drago H, Marin GH, Sturla F, Ibar R, Soratti C. Mesenchymal stem cells, could they be the link between tolerance and regeneration? Burns. 2007;33(2):137-8.
[5] Weinand C, Gupta R, Weinberg E, Madisch I, Neville CM, Jupiter JB, Vacanti JP. Toward regenerating a human thumb in situ. Tissue Eng Part A. 2009;15(9):2605-15.
[6] Potapov AG, Parmon VN. Biodegradable polymers – forward in the future. Ekologiia i Promyshlennost Rossii. 2010; (5 Sp iss):4–8.
[7] Jacquel N, Tajima K, Nakamura N, Kawachi H, Pan P, Inoue Y. Nucleation mechanism of polyhydroxybutyrate and poly(hydroxybutyrate- co -hydroxyhexanoate) crystallized by orotic acid as a nucleating agent . J Appl Polym Sci. 2010;115(2):709–15.
[8] Mauclaire L, Brombacher E, Bünger JD, Zinn M. Factors controlling bacterial attachment and biofilm formation on medium-chain-length polyhydroxyalkanoates (mcl-PHAs). Colloids Surf B Biointerfaces. 2010;76(1):104-11.
[9] Yu BY, Chen PY, Sun YM, Lee YT, Young TH. Effects of the surface characteristics of polyhydroxyalkanoates on the metabolic activities and morphology of human mesenchymal stem cells. J Biomater Sci Polym Ed. 2010;21(1):17-36.
[10] Shishatskaya EI, Volova TG, Gordeyev SA, Puzyr AP. Biodegradation of sutural threads on a basis of polyhydroxyalkanoates in biological environment. Perspectivnye Materialy. 2002; 2:56–62.
[11] Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials. 2005;26(33):6565-78.
[12] Brandl H, Gross RA, Lenz RW, Fuller RC. Plastics from bacteria and for bacteria: poly(beta-hydroxyalkanoates) as natural, biocompatible, and biodegradable polyesters. Adv Biochem Eng Biotechnol. 1990;41:77-93.
[13] Ciesielski S, Pokoj T, Klimiuk E. Molecular insight into activated sludge producing polyhydroxyalkanoates under aerobic-anaerobic conditions. J Ind Microbiol Biotechnol. 2008;35(8):805-14.
[14] Amass W, Amass A, Tighe B. A review of biodegradable polymers: uses, current developments in the synthesis and characterization of biodegradable polyesters, blends of biodegradable polymers and recent advances in biodegradation studies. Polym Int. 1998; 47(2):89–144.
[15] Dawes EA. Novel biodegradable microbial polymers. Dordrecht, Kluwer Acad. press, 1990; 287 p.
[16] Shishatskaya EI. Cellular matrixes from the resorbed polyhydroxyalkanoates. Cellular transplantology and tissue engineering. 2007; 2(2):68–75.
[17] Voĭnova ON, Kalacheva GS, Grodnitskaia ID, Volova TG. Microbial polymers as a degradable carrier for pesticide delivery. Prikl Biokhim Mikrobiol. 2009;45(4):427-31. .
[18] Livshits VA, Bonartsev AP, Jordansky AL, Ivanov EA, Mahina TA, Myshkina VL, Bonartseva GA. Mikrosfera from poly-3-hydroxybutirate for the prolonged release of medicinal substances. Vysokomolekulyarnyye soyedineniya. 2009; 51(7):1243–51.
[19] Shishatskaya EI, Goreva AV, Voinova ON, Inzhevatkin EV, Khlebopros RG, Volova TG. Evaluation of antitumor activity of rubomycin deposited in absorbable polymeric microparticles. Bull Exp Biol Med. 2008;145(3):358-61.
[20] Shishatskaia EI, Zhemchugova AV, Volova TG. Biodegradable polyhydroxyalkanoates as carriers for antitumor agents. Antibiot Khimioter. 2005;50(2-3):4-7.
[21] Shishatskaya EI, Kamendov IV, Starosvetsky SI, Volova TG. Research of osteoplastic properties of matrixes from resorbed polyether of hydroxybutyrate acid. Cellular transplantology and tissue engineering. 2008; 3(4):41–7.
[22] Ahmed T, Marçal H, Lawless M, Wanandy NS, Chiu A, Foster LJ. Polyhydroxybutyrate and its copolymer with polyhydroxyvalerate as biomaterials: influence on progression of stem cell cycle. Biomacromolecules. 2010;11(10):2707-15.
[23] Ke Y, Wang Y, Ren L. Surface modification of PHBV scaffolds via UV polymerization to improve hydrophilicity. J Biomater Sci Polym Ed. 2010;21(12):1589-602.
[24] Wang L, Wang ZH, Shen CY, You ML, Xiao JF, Chen GQ. Differentiation of human bone marrow mesenchymal stem cells grown in terpolyesters of 3-hydroxyalkanoates scaffolds into nerve cells. Biomaterials. 2010;31(7):1691-8.
[25] Deng Y, Zhao K, Zhang XF, Hu P, Chen GQ. Study on the three-dimensional proliferation of rabbit articular cartilage-derived chondrocytes on polyhydroxyalkanoate scaffolds. Biomaterials. 2002;23(20):4049-56.
[26] Zhao K, Deng Y, Chen GQ. Effects of surface morphology on the biocom-patibility of polyhydroxyalkanoates. Biochem Eng J. 2003; 16(2):115–123.
[27] Xu XY, Li XT, Peng SW, Xiao JF, Liu C, Fang G, Chen KC, Chen GQ. The behaviour of neural stem cells on polyhydroxyalkanoate nanofiber scaffolds. Biomaterials. 2010;31(14):3967-75.
[28] Dong Y, Li P, Chen CB, Wang ZH, Ma P, Chen GQ. The improvement of fibroblast growth on hydrophobic biopolyesters by coating with polyhydroxyalkanoate granule binding protein PhaP fused with cell adhesion motif RGD. Biomaterials. 2010;31(34):8921-30.
[29] Shishatskaya EI, Goreva AV, Voinova ON, Kalacheva GS, Volova TG. Distribution and resorption of polymeric microparticles in visceral organs of laboratory animals after intravenous injection. Bull Exp Biol Med. 2009;148(5):789-93.
[30] Shishatskaya EI, Volova TG Markelova NM, Vinnik YuS, Cherdantsev DV, Gavrilenko ES, Kuznetsov MN, Beletskii II, Zykova LD. The first results of a biodegraded sutural material based on linear polyether of 3-hydroxybutyrate acid application. Uspekhi sovremennogo yestestvoznaniya. 2008; 9:106–9.
[31] Fedorov MB, Vikhoreva GA, Kil'deeva NR, Mokhova ON, Bonartseva GA, Gal'braĭkh LS. Antimicrobial activity of core-sheath surgical sutures modified with poly-3-hydroxybutyrate. Prikl Biokhim Mikrobiol. 2007;43(6):685-90.
[32] Maiborodin IV, Shevela AI, Sheplev B.V, Kolesnikov IS, Matveeva VA, Drovosekov MN, Shevela AA, Kozodiy DM, Vybornov MS. Application biodegraded polyhydroxyalkanoates after injury of a bone of the bottom jaw to experiment. Clin Stomatol. 2010; 4:54–7.
[33] Maĭborodin IV, Shevela AI, Anishchenko VV, Matveeva VA, Shevela AA, Drovosekov MN, Vlasov VV. The peculiarities of rat tissue reactions to intraperitoneal implants made out of biodegradable polyhydroxyalkanoates. Morfologiia. 2011;139(2):62-6.
[34] Maiborodin IV, Shevela AI, Beregovoy EA, Matveeva VA, Angelsky AA, Drovosekov MN. The Intraarticulate implantation of biodegraded polyhydroxyalkanoates in experiment. Travmatologia I Ortopedia Rossii. 2011; 59(1):67–75.
[35] Boiandin AN, Kalacheva GS, Rodicheva EK, Volova TG. Synthesis of reserve polyhydroxyalkanoates by luminescent bacteria. Mikrobiologiia. 2008;77(3):364-9.
[36] Ruth K, Grubelnik A, Hartmann R, Egli T, Zinn M, Ren Q. Efficient production of (R)-3-hydroxycarboxylic acids by biotechnological conversion of polyhydroxyalkanoates and their purification. Biomacromolecules. 2007;8(1):279-86.
[37] Sun J, Dai Z, Zhao Y, Chen GQ. In vitro effect of oligo-hydroxyalkanoates on the growth of mouse fibroblast cell line L929. Biomaterials. 2007;28(27):3896-903.
[38] Volova TG, Kalacheva GS, Kozhevnikov IV, Steinbuchel A. Biosynthesis of multicomponent polyhydroxyalkanoates by Wautersia eutropha. Mikrobiologiia. 2007;76(6):797-804.
[39] Duan B, Wang M. Customized Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering: design, fabrication, surface modification and sustained release of growth factor. J R Soc Interface. 2010;7 Suppl 5:S615-29.
[40] Duan B, Wang M, Zhou WY, Cheung WL, Li ZY, Lu WW. Three-dimensional nanocomposite scaffolds fabricated via selective laser sintering for bone tissue engineering. Acta Biomater. 2010;6(12):4495-505.
[41] Li H, Zhai W, Chang J. Effects of wollastonite on proliferation and differentiation of human bone marrow-derived stromal cells in PHBV/wollastonite composite scaffolds. J Biomater Appl. 2009;24(3):231-46.
[42] Wang M, Chen LJ, Ni J, Weng J, Yue CY. Manufacture and evaluation of bioactive and biodegradable materials and scaffolds for tissue engineering. J Mater Sci Mater Med. 2001;12(10-12):855-60.