Biopolym. Cell. 2014; 30(4):299-304.
Molecular and Cell Biotechnologies
Antioxidant capacity and sustainability of Saccharomyces cerevisiae cells exposed to ozone
1Goriacha I. P., 1Zinchenko V. D., 1, 2Dyubko T. S., 3Romodanova E. A., 2Tatarets A. L.
  1. Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine
    23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015
  2. SSI "Institute for Single Crystals", NAS of Ukraine
    60, Lenin ave., Kharkiv, Ukraine, 61001
  3. National University of Pharmacy
    53, Pushkinska Str., Kharkiv, Ukraine, 61002


Aim. The investigation of response of S. cerevisiae cells to the effect of ozone in different doses and determination of total antioxidant capacity (TAC) of these cells. Methods. TAC was estimated with the chemiluminescent method when introducing ozone into S. cerevisiae cells. The damage of cells was determined by the methods of fluorescent microscopy and flow cytometry using fluorescent dye Square-460. Results. It was shown that under the ozone dosed administration into the S. cerevisiae cells suspension the chemiluminescence flash occurred in response to each new ozone doze. The flash amplitude slightly changes under low doses and decreases after achieving some total ozone dose, being characteristic for these cells. At the same time the damaged cells appear, the number of which augments with increasing the administered ozone dose. Conclusions. We assessed the TAC of S. cerevisiae cells, as the ozone dose, which may be neutralized by the systems of their antioxidant protection: 240 ± 20 nmol/106 cells. The S. cerevisiae cells are resistant to the effect of excessive ozone until its doses exceed the TAC value for these cells.
Keywords: oxidative stress, reactive oxygen species, ozone, S. cerevisiae, chemiluminescence.


[1] Belykh IA, Zinchenko VD, Vysekantsev IP. Stimulating effect of ozone low doses on microorganisms growth. Problems of Cryobiology (Problemy Kriobiologii). 2004; (4):41–6.
[2] Talbot P, Martinelli L, Talvy S, Chauveheid E, Haut B. Ozone inactivation of resistant microorganisms: Laboratory analysis and evaluation of the efficiency of plants. Water Res. 2012;46(18):5893-903.
[3] Hormesis: a revolution in biology, toxicology and medicine. Mattson M.P., Calabrese E.J. (Eds.). 2010. Humana Press Inc. 213 p.
[4] Pryor WA, Houk KN, Foote CS, Fukuto JM, Ignarro LJ, Squadrito GL, Davies KJ. Free radical biology and medicine: it's a gas, man! Am J Physiol Regul Integr Comp Physiol. 2006;291(3):R491-511.
[5] Bocci V. Ozone as Janus: this controversial gas can be either toxic or medically useful. Mediators Inflamm. 2004;13(1):3-11.
[6] Davies JM, Lowry CV, Davies KJ. Transient adaptation to oxidative stress in yeast. Arch Biochem Biophys. 1995;317(1):1-6.
[7] Zinchenko VD, Belykh IA, Buryak IA, Goriacha IP, Govor IV. Induction of the effects of crossed adaptation using oxidative stress to increase the efficiency of cryopreservation of biological objects. Actual problems of cryobiology and cryomedicine. Ed. by AN. Goltsev. Kharkov. 2012: 271–94.
[8] Karadag A, Ozcelik B, Saner S. Review of methods to determine antioxidant capacities. Food Anal Methods. 2009;2(1):41–60.
[9] Tirzitis G, Bartosz G. Determination of antiradical and antioxidant activity: basic principles and new insights. Acta Biochim Pol. 2010;57(2):139-42.
[10] Gerardi G, Usberti M, Martini G, Albertini A, Sugherini L, Pompella A, Di LD. Plasma total antioxidant capacity in hemodialyzed patients and its relationships to other biomarkers of oxidative stress and lipid peroxidation. Clin Chem Lab Med. 2002;40(2):104-10.
[11] Ramanathan B, Jan KY, Chen CH, Hour TC, Yu HJ, Pu YS. Resistance to paclitaxel is proportional to cellular total antioxidant capacity. Cancer Res. 2005;65(18):8455-60.
[12] Filippov JV, Voblikova VA, Panteleev VI. Electrosynthesis of ozone. M.: Moscow University Publishing House. 2008; 237 p.
[13] Lunin VV, Popovich MP, Tkachenko SN. Physical chemistry of ozone. M.: Moscow University Publishing House, 1998; 480 p.
[14] Pat. N 72111. Ukraine G01N 21. 76 (2006.01). Bioluminometer. Zinchenko V.D., Goriacha I.P., Govor I.V. An. 05.01.2012. Publ. 15.08.2012. Bul. N 15.
[15] Kohen R, Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol. 2002;30(6):620-50.
[16] Fulda S, Gorman AM, Hori O, Samali A. Cellular stress responses: cell survival and cell death. Int J Cell Biol. 2010;2010:214074.