Biopolym. Cell. 2014; 30(6):481-484.
Short Communications
Ca2+ does not affect the binding properties of ITSN1 EH domains
1Morderer D. Ye., 1Rymarenko O. V., 1Skrypkina I. Ya., 1Rynditch A. V.
  1. State Key Laboratory of Molecular and Cellular Biology
    Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


ITSN1 is an endocytic scaffold protein implicated in synaptic functioning. Ca2+ is known to be important for endo- cytosis in both pre- and post-synaptic terminals. ITSN1 contains two EH (Eps15 homology) domains which possess putative Ca2+-binding EF-hand motifs. Aim. To test the effect of Ca2+ on the EH domain binding properties. Methods. His-tag pulldown, Western blotting. Results. Addition of 1.5 mM Ca2+ does not affect the binding of the ITSN1 EH domains to the C-terminal fragment of the endocytic protein Epsin 1. Conclusions. The data obtained indicate that Ca2+ has no effect on the binding properties of the ITSN1 EH domains.
Keywords: ITSN1, Ca2+, EH domains


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