Biopolym. Cell. 2015; 31(3):226-232.
Viruses and Cell
Phylogenetic analysis of influenza A viruses (H3N2) circulating in Zhytomyr region during 2013–2014 epidemic season
1, 2Boyalska O. G., 3Kyrychuk I. M., 1Budzanivska I. G., 4Mironenko A. P., 4Radchenko L. V., 1Smutko O. Yu.
  1. Educational and Scientific Center "Institute of Biology"
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  2. SI "Zhytomyr regional laboratory center of State Sanitary and Epidemiological Service of Ukraine"
    64, V. Berdychivska Str., Zhytomyr, Ukraine, 10002
  3. Main Department of State Sanitary and Epidemiological Service of Ukraine in Zhytomyr region
    64, V. Berdychivska Str., Zhytomyr, Ukraine, 10002
  4. SI "Gromashevsky L. V. Institute of epidemiology and infectious diseases, NAMS of Ukraine"
    5, Amosova Str., Kyiv, Ukraine, 03038


Aim. To perform phylogenetic analysis of the hemagglutinin (HA) and neuraminidase (NA) genes of influenza A(H3N2) viruses circulating in the Zhytomyr region during 2013–2014 epidemic season. To make comparison of the HA and NA genes sequences of the Zhytomyr region isolates with the HA and NA genes sequences of influenza viruses circulating in the world. Methods. Laboratory diagnosis was conducted by real-time polymerase chain reaction (RT-PCR). In this study the sequencing and phylogenetic analysis were carried out. Results. For the first time the genes of influenza A(H3N2) viruses isolated in the Zhytomyr region during 2013–2014 epidemic season, coding hemagglutinin and neuraminidase were compared with their orthologs. According to the results of this comparison the phylogenetic tree was constructed. Additionally, the amino acid substitutions of the influenza viruses circulating in Ukraine and worldwide were analyzed. Conclusions. The nucleotide sequences of the influenza A(H3N2) viruses genes HA and NA isolated in the Zhytomyr region were identified. Based on the nucleotide sequences of HA and NA we constructed the influenza virus phylogenetic tree demonstrating that the virus isolated in the Zhytomyr region was closely related to the Ukrainian isolate from Kharkov and in the world to the isolates from Germany, Romania, Italy.
Keywords: A(H3N2) influenza viruses, phylogenetic analysis, epidemic season, mutations.


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