Biopolym. Cell. 2015; 31(6):458-464.
Molecular Biomedicine
Aberrant expression of metallothioneins in clear cell renal cell carcinomas
1Rymar V. I., 1Lototskaya L. V., 1Kondratov O. G., 2Stakhovsky E. O., 2Kononenko O. A., 1Kashuba V. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. National Cancer Institute
    33/43, Lomonosova Str., Kyiv, Ukraine, 03022


Aim. To find candidate tumor suppressor genes among metallothioneins for clear cell renal cell carcinoma. Methods. Analysis of the microarray data, quantitative PCR. Results. We found three genes encoding metallothioneines that showed reduced expression in different types of renal tumors, using protocol of the cross-platform meta-analysis of microarray data with normalization on several reference genes. Decreased expression of the MT1G, MT1F, and MT1H genes in clear cell renal cell carcinoma was confirmed by qPCR. Conclusions. The MT1G, MT1F and MT1H genes as well as may be considered as the candidate tumor suppressor genes for ccRCC.
Keywords: metallothionein, renal tumors, candidate tumor suppressor gene, microarrays


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