Biopolym. Cell. 2016; 32(2):131-139.
Viruses and Cell
Extreme evolutionary stability of conserved non-protein coding element of baculovirus genome
1Makarenko V. E., 1Kikhno I. M., 1Kashuba V. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680


Aim. To estimate a level of evolutionary stability of the baculovirus conserved non protein-coding element (CNE) playing an essential role in baculovirus pathogenesis. Methods. NCBI-BLAST was applied to identify the orthologous sequences in genomes of 50 alphabaculoviruses. The orthologous sequences were aligned by using ClustalW software. Alistat tool was applied to obtain the pairwise percent identity ( %ID) matrix data. Results. An average pairwise ID value (73 %) calculated for the 1225-member sample was shown to be comparable with those of coding parts of the most conserved baculovirus genes polh and pif2 as well as with those of the polh and p18 promoter regions which are the most conserved representatives of a small group of the evolutionary stable promoter regions of the alphabaculovirus late genes. Conclusion. CNE is one of the most conserved elements of the alphabaculovirus genome.
Keywords: Baculovirus, conserved non-coding element


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