Biopolym. Cell. 2018; 34(1):24-31.
Molecular and Cell Biotechnologies
Characterization of vaccine and field IBDV strains in Ukraine for proper vaccine selection for disease prevention
1Pastyria A. S., 1Budzanivska I. G., 1Polischuk V. P.
  1. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601


Infectious bursal disease virus (IBDV) causes a highly contagious disease in young chickens and is distributed worldwide. Primary viral antigen is VP2. The VP2 gene contains hypervariable re-gion (VP2 HRV). Mutations in this region lead to emergence of antigenically different IBDV strains. For IBDV prevention, vaccination is used. The efficacy of vaccination depends on the ge-netic closeness of field and vaccine strains. Aim of the study was to analyze nucleotide sequence of different vaccine and field strains of IBDV circulating in Ukrainian poultry farms. Methods. In this study 11 vaccine strains and 16 field isolates were used. RNA was extracted using a magnetic separation method, reverse transcription was carried out and PCR was performed using specific primers to the VP2 gene. Obtained amplicons were used for sequencing. Phylogenetic and amino acid analysis was performed with MEGA 6 software. Results. 11 vaccine strains formed 5 phy-logenetic clusters. Cluster I represented strains GM97, 228E and MB/20. Cluster II contained mild vaccine strains LC-75 and D78. Intermediate strains Winterfield-2512 and Lukert formed cluster III. ‘Hot’ vaccine strains MB and MB/3 formed cluster IV. Cluster V was represented by strains MB/5 and V877. After addition of 16 Ukrainian field strains the tree structure remained the same. 8 isolates clustered together with ‘hot’, 5 – with intermediate, and 3 – with mild vaccine strains. Amino acid analyses confirmed antigenic closeness among vaccine and field strains of the same cluster. Conclusion. The obtained data can be used for the vaccine selection for IBD pre-vention in each particular poultry farm of Ukraine.
Keywords: IBDV, field strains, vaccine strains, vvIBDV, phylogenetic analysis.


[1] Berg TP. Acute infectious bursal disease in poultry: a review. Avian Pathol. 2000;29(3):175-94.
[2] Alkie TN, Rautenschlein S. Infectious bursal disease virus in poultry: current status and future prospects. Veterinary Medicine: Research and Reports. 2016; 2016(7):9–18.
[3] Jackwood DJ, Sreedevi B, LeFever LJ, Sommer-Wagner SE. Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity. Virology. 2008;377(1):110-6.
[4] Camilotti E, Moraes LB, Furian TQ, Borges KA, Moraes HLS, Salle CTP. Infectious Bursal Disease: Pathogenicity and Immunogenicity of Vaccines. Rev Bras Cienc Avic. 2016; 18(2):303–8.
[5] Jackwood DJ. Advances in vaccine research against economically important viral diseases of food animals: Infectious bursal disease virus. Vet Microbiol. 2017;206:121-125.
[6] Boudaoud A, Mamache B, Tombari W, Ghram A. Virus mutations and their impact on vaccination against infectious bursal disease (Gumboro disease). Rev Sci Tech. 2016;35(3):875-897.
[7] Pastyria A, Sobko I, Polischuk V. Genetic characterization of infectious bursal disease virus isolates in Ukraine. Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology. 2016; 72(2):24–27.
[8] Kataria RS, Tiwari AK, Bandyopadhyay SK, Kataria JM, Butchaiah G. Detection of infectious bursal disease virus of poultry in clinical samples by RT-PCR. Biochem Mol Biol Int. 1998;45(2):315-22.
[9] Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013;30(12):2725-9.
[10] Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-25.
[11] Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39(4):783-791.
[12] Mawgod SA, Arafa AS, Hussein HA. Molecular genotyping of the infectious bursal disease virus (IBDV) isolated from Broiler Flocks in Egypt. Int J Vet Sci Med. 2014; 2(1):46–52
[13] Bayliss CD, Spies U, Shaw K, Peters RW, Papageorgiou A, Müller H, Boursnell ME. A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. J Gen Virol. 1990;71 ( Pt 6):1303-12.
[14] Mohamed MA, Elzanaty KES, Bakhit BM, Safwat MM. Genetic Characterization of Infectious Bursal Disease Viruses Associated with Gumboro Outbreaks in Commercial Broilers from Asyut Province, Egypt. ISRN Vet Sci. 2014; 2014(1):916412.
[15] Jackwood DJ, Sommer-Wagner SE. Amino acids contributing to antigenic drift in the infectious bursal disease Birnavirus (IBDV). Virology. 2011;409(1):33-7. d