Biopolym. Cell. 2020; 36(1):23-35.
Molecular Biomedicine
Cytokines profile in knee cartilage of rats during monoiodoacetate-induced osteoarthritis and administration of probiotic
1Korotkyi O. H., 1Vovk A. A., 1Halenova T. I., 1Vovk T. B., 1Dvorshchenko K. O., 1Falalyeyeva T. M., 1Ostapchenko L. I.
  1. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601


Aim. To identify the effect of probiotic (PB) on cytokines profile in knee cartilage of rats with experimental osteoarthritis (OA). Methods. The animals of 1st (Control) and 3rd (PB) groups got 50 µL of saline to both hind knees. The animals of 2nd (MIA-OA) and 4th groups (MIA-OA-PB) got single injection of 1 mg monoiodoacetate (MIA) dissolved in 50 µL of saline to knee, after which MIA-OA developed for 28 days. PB feeding [was] provided daily for 14 days during the MIA-OA progress. The levels of cytokines (IL-1β, TNF-α, IL-6, IL-8, IFN-γ, IL-4, IL-10, TGF-β, IGF-1) were measured in cartilage homogenates by [the] enzyme-linked immunosorbent assay (Biotrak ELISA System, GE Healthcare, USA). Results. MIA-OA caused [an] increase in the levels of IL-1β, TNF-α, IL-6, IL-8, IFN-γ, IL-10, TGF-β, decreased the level of IGF-1 and did not change the level of IL-4, compared to Control. PB during MIA-OA increased the level of IGF-1, decreased the levels of IL-1β, TNF-α, IL-6, IL-8, IFN-γ, TGF-β, compared to MIA-OA, but did not reach the Control values (unlike IL-4 and IL-10 that equaled to Control). Conclusions. MIA-OA caused significant changes in the levels of studied cytokines in knee cartilage. An application of PB has positive local anti-inflammatory effect in cartilage tissue of rats with MIA-OA.
Keywords: osteoarthritis, cytokines, growth factors, probiotic, cartilage


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