Biopolym. Cell. 2023; 39(3):220-230.
Molecular Biomedicine
Effect of complexes of natural oligoribonucleotides with D-mannitol on the viability of cell cultures of different origin
1Kraievska I. M., 1Tkachuk Z. Yu.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143


Aim. Investigation of the effect of acidic and salt forms of natural yeast RNA oligoribonucleotides and their complexes with D-mannitol (ORN-D-M) on the viability of cell cultures of different origins. Methods. MTT assay, differential trypan blue staining. Results. A dose-dependent inhibition of the viability of mouse melanoma B16 cells and human glioblastoma U251 cells under the influence of ORN-D-M was established, as well as a dependence on the treatment time. U251 cells were more sensitive to the effect of the drug: at 48-hour treatment, the IC50 for this cell line was 1.0±0.3 mg/ml, whereas for B16 it was 2.5±0.4 mg/ml. At the same time, the inhibition of non-malignant canine kidney MDCK cells was observed only at high concentrations of the drug (IC50 – 6.1±1.1 mg/ml). The acidic form of ORN-D-M inhibited the viability of B16 and U251 cell cultures whereas the acidic form of ORN, salt forms of Na-ORN and Na-ORN-D-M showed a significantly lower inhibition activity. Conclusions. The acidic form of ORN-D-M has significant inhibitory activity against the malignant cells of mouse melanoma B16 and human glioblastoma U251. The acidic form of ORN-D-M is a promising drug for further antitumor studies in vitro and in vivo.
Keywords: natural oligoribonucleotides, ORN-D-M, anticancer activity, MTT assay


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