Biopolym. Cell. 1988; 4(2):91-100.
Cell Biology
Nocodazole inactivates microtubule organizing centres and induces the formation of local zones of microtubule polymerization in k-mitosis
1Alieva I. B., 1Vorobjev I. A.
  1. A. N. Belozersky Interfaculty Laboratory of Molecular Biology and Bioorganic Chemistry,
    M. V. Lomonosov State University
    Moscow, USSR


Under the action of nocodasole at a concentration of 0.02 μg/ml the mitotic spindle is preserved in PE cells. Nocodazole at a concentration of 0.2 μg/ml results in the appearance of microtubule (MT) convergence centres lacking the centrioles and/or chromosomes. Bundles of MT bypass kinetochores, kinetochores become 1.5–3-times as long. At a nocodazole concentration of 0.02–0.2 μg/ml the daughter centriole becomes 1.5–2-fold as long. Under the action of nocodazole at a concentration of 0.6 μg/ml all chromosomes are packed in a slot-like structure. Kinetochores of several chromosomes surrounding one «slot» may be situated on their inner surfaces. Then short disorderly MT are observed in such a «slot». The centrioles are not surrounded by MT. In each cell one diplosome (rarely both) disintegrate. It is supposed that the appearance of three- and tetrapolar mitoses in tissue culture cells is due to the disintegration of one or both diplosomes, respectively.


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