Biopolym. Cell. 1988; 4(5):250-254.
Structure and Function of Biopolymers
The efficient method for DNA encapsulation into reconstituted sendai virus envelopes
1Vlassov V. V., 1Krendelev Yu. D., 2Ovander M. N., 1Ryte A. S., 1Repin V. E., 1Svinarchuk F. P.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR
  2. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


The freezing-thawing method is used for encapsulation up to 17000 base pairs DNA into reconstituted Sendai virus envelopes (RSVE). It permits encapsulating up to 30 % of DNA added to the solution. The interaction RSVE loaded with plasmid DNA and Krebs-2 ascites tumours cells results in up to 30 % penetration of the encapsulated material into the cells.


[1] Celis JE. Microinjection of somatic cells with micropipettes: comparison with other transfer techniques. Biochem J. 1984;223(2):281-91.
[2] Anderson WF. Prospects for human gene therapy. Science. 1984;226(4673):401-9.
[3] Cepko CL, Roberts BE, Mulligan RC. Construction and applications of a highly transmissible murine retrovirus shuttle vector. Cell. 1984;37(3):1053-62.
[4] Vainstein A, Razin A, Graessmann A, Loyter A. Fusogenic reconstituted Sendai virus envelopes as a vehicle for introducing DNA into viable mammalian cells. Methods Enzymol. 1983;101:492-512.
[5] Hsu MC, Scheid A, Choppin PW. Reconstitution of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution. Virology. 1979;95(2):476-91.
[6] Harmsen MC, Wilschut J, Scherphof G, Hulstaert C, Hoekstra D. Reconstitution and fusogenic properties of Sendai virus envelopes. Eur J Biochem. 1985;149(3):591-9.
[7] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. New York, Cold Spring Harbor, 1982; 545 p.
[8] Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265-75.
[9] Citovsky V, Blumenthal R, Loyter A. Fusion of Sendai virions with phosphatidylcholine-cholesterol liposomes reflects the viral activity required for fusion with biological membranes. FEBS Lett. 1985;193(2):135-40.