Biopolym. Cell. 2024; 40(1):58-67.
http://dx.doi.org/10.7124/bc.000AAE
Molecular and Cell Biotechnologies
Effect of light and temperature on the content of some biologically active substances in Deschampsia antarctica tissue culture
1Twardovska M. O., 1Konvalyuk I. I., 2Lystvan K. V., 1Kunakh V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03143

Abstract

Aim. The aim of the work was to study the influence of growth conditions (darkness/light and temperature 18°/26°C) on the content of phenolic compounds and flavonoids in D. antarctica morphogenic tissue culture. Methods. In vitro tissue culture, Folin-Ciocalteu method, spectrophotometry, HPLC analysis. Results. The total content of phenolic compounds and flavonoids in D. antarctica morphogenic tissue cultures, obtained from the plants genotypes DAR12 and G/D11-1/3 was determined. It was shown that in growth calli the light intensity of 6500 lux and raised temperature of 26°C led to a decrease in the content of biologically active substances (BAS). When culturing calli of both genotypes in the darkness, regardless of the temperature, the level of phenolic compounds (2 times for DAR12 and 2.8–3.1 times for G/D11-1/3) as well as of flavonoids (2.3–2.4 times for DAR12 and 4.6–5 times for G/D11-1/3) decreased. The antioxidant and antitumor compound tricin was found, the content of which was three times higher in the initial plant DAR12 compared to the G/D11-1/3 plant. The content of tricin in the calli was lower than in the initial plants. Conclusions. It was found that the highest level of the BAS accumulation in the morphogenic tissue culture of D. antarctica was observed when it was growing in the light intensity of 6500 lux and at the temperature of 18°C. The tricin detection in the initial plants of genotypes DAR12 and G/D11-1/3, as well as in the tissue cultures provides a basis for further biochemical study of D. antarctica in vitro as a potential source of BAS, which can be used for therapeutic and prophylactic purposes.
Keywords: Deschampsia antarctica E. Desv., plant tissue culture, phenolic compounds, flavonoids, tricin
Full text: (PDF, in English)

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